摘要：

为培育优质专用小麦,需要从育种早代开始品质参数的选择,由于在育种早代种子数量有限,只能选用一些小量品质测定方法。在常用的小量品质测定方法中,麦谷蛋白溶胀指数(SIG)和乳酸SDS溶剂保持力(LA-SDS SRC)2种方法预测小麦品质的效果比较好,目的是对这2种方法进行比较和必要的优化,为提高品质育种效率提供支持。以黄淮麦区培育的8个强筋、中强筋和中筋小麦品种为试验材料,用全麦粉分别测定不同材料的SIG和LA-SDS SRC值并对试验结果进行了比较,并对LA-SDS SRC法进一步做了优化处理。结果表明,SIG和LA-SDS SRC法测定的结果均与这些品种的面筋强度顺序一致,比较而言,LA-SDS SRC法预测面筋强度效果更好,能更好体现强筋品种与中筋品种之间的差异;优化后的LA-SDS SRC法(使用2 mL离心管)与标准LA-SDS SRC法(使用50 mL离心管)的测定结果高度相关,相关系数高达0.986,其预测面筋强度的效果优于使用2 mL离心管的SIG法。标准的LA-SDS SRC法和更小量的LA-SDS SRC法适合作为我国小麦品质育种的早代选择方法。

关键词: 小麦, 加工品质, 微量测定方法, SIG, LA-SDS SRC

Abstract:

In order to cultivate a specific type of high-quality wheat,it is necessary to make the quality measurement from the early generation. Due to the limitation of the seed amount in the early generation,only some small measurement methods can be used. Among the commonly used small quantity of quality measurement methods,glutenin swelling index (SIG)and lactate SDS solvent retention capacity (LA-SDS SRC)are better methods to predict wheat quality. The purpose of this study was to compare these two methods and make necessary optimizations,so as to provide support for improving quality breeding efficiency. This study used 8 strong gluten,medium-strong gluten,and medium gluten wheat varieties cultivated in the Huanghuai wheat region as experimental materials. The SIG and LA-SDS SRC values of different materials were measured using whole wheat flour,and the experimental results were compared. The LA-SDS SRC method was further optimized. The results showed that the results measured by SIG and LA-SDS SRC methods were consistent with the order of gluten strength of these varieties. In comparison,LA-SDS SRC method had a better prediction effect on gluten strength and could better reflect the differences between strong gluten varieties and medium gluten varieties;the optimized LA-SDS SRC method (using a 2 mL centrifuge tube)showed a high correlation with the standard LA-SDS SRC method (using a 50 mL centrifuge tube),with a correlation coefficient of up to 0.986. Its predictive effect on gluten strength was superior to the SIG method using a 2 mL centrifuge tube. The standard LA-SDS SRC method and a smaller amount of LA-SDS SRC method are suitable as early generation selection methods for wheat quality breeding in China.

Key words: Wheat, Processing quality, Micro-determination method, SIG, LA-SDS SRC