摘要： 为改善葡萄果实着色效果，探讨日光温室内ALA处理最佳浓度。在弗雷无核葡萄果实膨大期和着色期，利用50，100，150 mg/L的5-氨基乙酰丙酸（ALA）溶液喷布果实，研究外源ALA处理对葡萄果实外观色泽、内在品质及花青素合成相关酶活性的影响。结果表明：在果实成熟过程中，外源ALA处理均能诱导果皮花青素的积累，促进果皮着色，缓解果皮叶绿素和类胡萝卜素的降解速率；果实可溶性糖含量显著提高，可滴定酸含量明显下降。ALA处理后果皮花青素含量与PAL活性极显著相关，与DFR活性均无显著相关性，CHI活性仅在处理后与果皮花青素含量极显著相关，但经ALA处理后，果皮PAL、CHI、DFR活性较对照均有不同程度的提升，由此推测，ALA通过上调相关调控因子的表达来诱导花青素合成积累，从而改善果实外观及内在品质。

关键词: 5-氨基乙酰丙酸(ALA), 葡萄, 果皮着色, 品质, PAL, CHI, DFR

Abstract: In order to improve the effect of grape fruit coloring,the optimum concentration of ALA in solar greenhouse was discussed. In the enlargement and coloring period of the Flame seedless fruit,the use of 5-aminolevulinic acid 50,100,150 mg/L(ALA) spraying fruit,to investigate the effects of ALA treatment on color,quality and anthocyanin synthesis related enzyme activity of grape fruit appearance. The results showed that during the fruit ripening,the treatment of exogenous ALA could induce the accumulation of anthocyanin,promote the colouring of peel,and alleviate the degradation rate of chlorophyll and carotenoids; The content of soluble sugar in fruit increased significantly,and the content of titration decreased obviously. After treatment with ALA,the anthocyanin content of the peel was significantly correlated with PAL activity,there was no significant correlation with DFR activity,CHI activity was significantly related only after the treatment and the content of anthocyanin,but after treatment of ALA,PAL,CHI,DFR activity in pericarp than control had different degrees of improvement,by hypothesizing that the expression of ALA by upregulating the related regulation the factor to induce synthesis of anthocyanin accumulation,thereby improving fruit appearance and internal quality.

Key words: 5-aminolevulinic acid (ALA), Grape, Pericarp coloring, Quality, PAL, CHI, DFR

中图分类号: 

• S663.01