华北农学报 ›› 2016, Vol. 31 ›› Issue (6): 39-43. doi: 10.7668/hbnxb.2016.06.007

所属专题: 小麦 生物技术

• 论文 • 上一篇    下一篇

Puroindoline b位点近等基因系对小麦籽粒淀粉含量和组分及其特性的影响

秦海霞1, 侯俊峰1, 丁会纳1, 夏先春2, 何中虎2, Morris F Craig3, 王晨阳1, 马冬云1   

  1. 1. 河南农业大学 农学院, 国家小麦工程技术研究中心, 河南 郑州 450002;
    2. 中国农业科学院 作物科学研究所, 国家小麦改良中心, 北京 100081;
    3. 美国农业部农业研究中心, 西部小麦品质实验室, 普尔曼 99164-6494
  • 收稿日期:2016-06-02 出版日期:2016-12-28
  • 作者简介:秦海霞(1990-),女,河南洛阳人,硕士,主要从事小麦品质生理研究。
  • 基金资助:
    国家自然科学基金项目(31571651);省部共建小麦玉米作物学国家重点实验室自主课题(39990035)

Starch Content and Properties of Common Wheat in Near-isogenic Lines of Puroindoline b Alleles

QIN Haixia1, HOU Junfeng1, DING Huina1, XIA Xianchun2, HE Zhonghu2, Morris F Craig3, WANG Chenyang1, MA Dongyun1   

  1. 1. Agronomy College of Henan Agricultural University, National Engineering Research Center for Wheat, Zhengzhou 450002;
    2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Wheat Improvement Center, Beijing 100081, China;
    3. USDA-ARS, Western Wheat Quality Laboratory, Pullman WA99164-6494, USA
  • Received:2016-06-02 Published:2016-12-28

摘要: 为了研究不同硬度等位基因与小麦籽粒淀粉组分含量及特性的关系,为小麦品质改良提供依据,以7个硬度Puroindoline b位点近等基因系为试材,研究了不同硬度基因型对小麦籽粒淀粉组分含量及特性的影响。结果表明,Pina-D1b/Pinb-D1a基因型的籽粒硬度值、支链淀粉含量最高;Pina-D1b/Pinb-D1a基因型的淀粉溶解度最高,而Pina-D1a/Pinb-D1d溶解度最低,两者差异达显著水平;不同基因型之间籽粒淀粉酶解力没有显著差异;冻融失水率以基因型Pina-D1a/Pinb-D1b最低,表明Pina-D1a/Pinb-D1b基因型冻融稳定性最好,可能较适宜冷冻食品的制作;对于抗性淀粉,Pina-D1a/Pinb-D1b基因型的抗性淀粉含量最高、Pina-D1a/Pinb-D1d最低,表明Pina-D1a/Pinb-D1b基因型有助于籽粒中抗性淀粉含量的提高。

关键词: 小麦, Puroindoline基因, 近等基因系, 淀粉特性, 淀粉含量

Abstract: Understanding the effect of Puroindoline b (Pinb) alleles on wheat grain starch content and properties will lay the foundation for improving the wheat quality.Seven wheat near-isogenic lines (NILs) differing in Puroindoline b were used to compare their grain starch component content and properties.The results showed that Pina-D1b/Pinb-D1a genotype possessed significantly higher values of grain hardness and amylopectin content than other genotypes.The Pina-D1b/Pinb-D1a genotype possessed the highest starch solubility,and the Pina-D1a/Pinb-D1d genotype had the lowest value,their difference being significant.There was no significant difference in starch enzymolysis force between different genotypes.The Pina-D1a/Pinb-D1b genotype had the lowest freezing and thawing loss rate,indicating that Pina-D1a/Pinb-D1b genotype possessed better freeze-thaw stability and would suit for making frozen food.For resistant starch,the Pina-D1a/Pinb-D1b genotype possessed the highest resistant starch content,while the lowest value went to Pina-D1a/Pinb-D1d,which suggested that Pina-D1a/Pinb-D1b genotype contributes to high resistant starch content of grains.

Key words: Wheat, Puroindoline gene, Near-isogenic lines, Starch properties, Starch content

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引用本文

秦海霞, 侯俊峰, 丁会纳, 夏先春, 何中虎, Morris F Craig, 王晨阳, 马冬云. Puroindoline b位点近等基因系对小麦籽粒淀粉含量和组分及其特性的影响[J]. 华北农学报, 2016, 31(6): 39-43. doi: 10.7668/hbnxb.2016.06.007.

QIN Haixia, HOU Junfeng, DING Huina, XIA Xianchun, HE Zhonghu, Morris F Craig, WANG Chenyang, MA Dongyun. Starch Content and Properties of Common Wheat in Near-isogenic Lines of Puroindoline b Alleles[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 39-43. doi: 10.7668/hbnxb.2016.06.007.