水稻窄卷叶突变体nrl<sub>（t）</sub>的遗传分析与基因定位

doi:10.7668/hbnxb.2014.04.007

摘要/Abstract

摘要： 为发掘、定位和克隆水稻窄卷叶相关突变基因，揭示叶片发育机理。对武运粳7号条纹叶枯病抗性改良品系中发现的窄卷叶突变体进行了表型观察、遗传分析及基因定位。结果表明，与野生型相比，突变体在叶片形态发生改变的同时，其株高、分蘖性、穗长、枝梗数、每穗实粒数、穗粒数、结实率、千粒质量等农艺性状也存在显著差异。遗传分析表明，该突变性状受一对隐性基因控制。以500株辐恢838/nrl_（t）的F₂隐性单株为定位群体，利用SSR标记将 NRL_（t）基因定位于水稻第11染色体短臂末端RM11-01、RM11-11之间，物理距离约为160 kb的范围内。通过水稻基因组注释数据库分析发现，在该区域存在26个预测的基因，测序分析表明已报道的 homeobox3A 基因可能是潜在的候选基因，这为进一步研究该基因奠定了基础。

关键词: 水稻, 窄卷叶, 突变体, 遗传分析, 基因定位

Abstract: Leaf morphology is one of key components for rice plant architecture,so excavation,mapping and cloning related mutant genes are very important for revealing mechanism of leaf development and breeding new rice varieties with ideotype and super-high yield.In this study,the narrow and rolled mutant ntl_(t) was found from the improved line of rice stripe disease for Wuyunjing No.7 and the mutant phenotype genetic analysis and gene mapping was researched.Compared with wild type,the mutant showed multipe abnormal phenotypes,such as shorter leaf length and width,rolled leaf,dwarfism,more tillers,reduced panicle length,branch number,filled grain number per spike,spikelets per spike,seed-setting rate and 1000-grain weight.Genetic analysis indicated that the mutant was controlled by a single recessive gene.Using 500 recessive mutant individuals in the F₂ population derived from the cross between Fuhui 838 and nrl_(t),the gene NRL _(t) was located to a confined region of 160 kb flanked by two microsatellite markers RM11-01 and RM11-11 on the short arm of chromosome 11.Based on the annotation result of rice genome,26 open reading frames(ORFs)were predicted in this region.Sequencing analysis showed that the homeobox3A gene reported might be the potential candidate gene.These results are very valuable for further study on this gene.

Key words: Oryza sativa L., Narrow and rolled leaf, Mutant, Genetic analysis, Gene mapping

中图分类号:

陈涛, 刘燕清, 张亚东, 朱镇, 赵庆勇, 周丽慧, 姚姝, 于新, 赵凌, 王才林. 水稻窄卷叶突变体nrl_（t）的遗传分析与基因定位[J]. 华北农学报, 2014, 29(4): 37-43. doi: 10.7668/hbnxb.2014.04.007.

CHEN Tao, LIU Yan-qing, ZHANG Ya-dong, ZHU Zhen, ZHAO Qing-yong, ZHOU Li-hui, YAO Shu, YU Xin, ZHAO Ling, WANG Cai-lin. Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl_(t) in Rice[J]. journal1, 2014, 29(4): 37-43. doi: 10.7668/hbnxb.2014.04.007.

http://www.hbnxb.net/CN/Y2014/V29/I4/37

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水稻窄卷叶突变体nrl_（t）的遗传分析与基因定位

Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl_(t) in Rice

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水稻窄卷叶突变体nrl（t）的遗传分析与基因定位

Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl(t) in Rice

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水稻窄卷叶突变体nrl_（t）的遗传分析与基因定位

Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl_(t) in Rice