光对蔬菜硝酸盐累积的影响及其机理

doi:10.7668/hbnxb.2011.S2.029

摘要/Abstract

摘要： 硝酸盐是一类极易在蔬菜特别是叶菜类蔬菜中高量累积的物质,过量摄入硝酸盐对人体健康存在极大的潜在危害。光照是影响植物硝酸盐累积最重要的环境因子之一,笔者分别从光强、光质、光照时间3个方面综述了光对蔬菜硝酸盐累积的影响,阐述了传统的昼夜交替光照模式下蔬菜硝酸盐含量的日变化规律,并着重从硝酸盐吸收和同化的角度综合阐述了光照影响植物硝酸盐累积的机理。

关键词: 光, 硝酸盐, 硝酸盐代谢, 硝酸还原酶

Abstract: Nitrate is a type of substance easily accumulated in vegetables,especially in leaf vegetables,and poses serious health hazard if ingested excessively.Light is one of the most important environmental factors that influence nitrate content in plants.This review summarized the effects of light on nitrate accumulation in vegetables from the aspect of light intensity,light quality and light duration respectively,expatiated the daily variation in nitrate content in vegetables under traditional lighting mode,and summarized the effect mechanism from the aspect of nitrate absorption and assimilation.

Key words: Light, Nitrate, Nitrate metabolism, Nitrate reductase

中图分类号:

Q945.1

周晚来, 刘文科, 杨其长. 光对蔬菜硝酸盐累积的影响及其机理[J]. 华北农学报, 2011, 26(S2): 125-130. doi: 10.7668/hbnxb.2011.S2.029.

ZHOU Wan-lai, LIU Wen-ke, YANG Qi-chang. Effect of Light on Nitrate Accumulation in Vegetables and the Mechanism Thereof[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S2): 125-130. doi: 10.7668/hbnxb.2011.S2.029.

http://www.hbnxb.net/CN/Y2011/V26/IS2/125

参考文献

[1] Gangolli S D,Van den Brandt P A,Feron V J,et al.Assessment of nitrate,nitrite,and N-nitroso compounds[J].European Journal of Pharmacology:Environmental Toxicology and Pharmacology,1994,292:1-38.
[2] Eichholzer M,Gutzwiller F.Dietary nitrates,nitrites,and N-Nitroso compounds and cancer risk:a review of the epidemiologic evidence[J].Nutrition Review,1998,56:95-105.
[3] Mensinga T T,Speijers G J A,Meulenbelt J.Health implications of exposure to environmental nitrogenous compounds[J].Toxicological Reviews,2003,22:41-51.
[4] Santamaria P.Review nitrate in vegetables:toxicity,content,intake and EC regulation[J].Journal of the Science of Food and Agriculture,2006,86:10-17.
[5] 高祖民,李式军,索长江,等.不同光强下硝酸态氮与钼对叶菜类硝酸盐积累的影响[J].园艺学报,1987,14(3):192-196.
[6] 周根娣,卢善玲.磷钾肥、光照、贮藏加工对蔬菜硝酸盐含量的影响[J].上海农业学报,1991,7 (2):53-56.
[7] 王忠全,吴志鹏,周子平.氮源配比及光强对水培蔬菜生长和营养品质的影响[J].上海蔬菜,2005,1:70-71.
[8] 周秋月,吴沿友,许文祥,等.光强对生菜硝酸盐累积的影响[J].农机化研究,2009,1:189-192.
[9] 周泽义,胡长敏,王敏健,等.中国蔬菜硝酸盐和亚硝酸盐污染因素及控制研究[J].环境科学进展,1999,7(5):1-13.
[10] 熊国华,林咸永,章永松,等.环境因素对蔬菜累积硝酸盐影响的研究进展[J].土壤通报,2004,35(3):362-365.
[11] 傅志坚,罗安程.设施栽培蔬菜硝酸盐积累问题[J].浙江农业科学,2004,2:80-82.
[12] 齐连东,刘世琦,许莉,等.光质对菠菜草酸、单宁及硝酸盐积累效应的影响[J].农业工程学报,2007,23(4):201-205.
[13] 徐莉.光质对叶用莴苣生理特性及品质的影响[D].山东:山东农业大学,2007.
[14] Akvile U,Paulo P,Giedre S,et al.Effect of short-wavelength light on lettuce growth and nutritional quality[J].Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture,2007,26(1):157-165.
[15] 闻婧,鲍顺淑,杨其长,等.LED 光源R/B对叶用莴苣生理性状及品质的影响[J].中国农业气象,2009,30(3):413-416.
[16] Steingrover E G,Siesling J,Ratering P.Effect of one night with "low light" on uptake,reduction and storage of nitrate in spinach[J].Physiol Plant,1986,66:557-562.
[17] 蒋自立.农作物积累硝酸盐的农业生态因素[J].农业环境与发展,1991,1:24-26.
[18] Hageman R H,Flesher D,Giller H.Diurnal variation and other light effects influencing the activity of nitrate reductase and nitrogen metabolism in corn[J].Crop Sci,1961,1:201-204.
[19] M inotti P L,Stankey D L.Diurnal variation in the nitrate concentration of beets[J].Hortscience,1973,8:33-34.
[20] 林志刚,赵仪华,薛耀英.叶菜类蔬菜的硝酸盐积累规律及其控制方法研究[J].土壤通报,1993,24(6):253-255.
[21] Carrasco G A,Burrage S W.Diurnal fluctuations in nitrate uptake and nitrate accumulation in lettuce(Lactuca sativa L.)[J].Acta Horticultura,1993,339:137-147.
[22] Scaife A,Schloemer S.The diurnal pattern of nitrate uptake and reduction by spinach (Spinacia oleracea L.)[J].Annals of Botany,1994,73:337-343.
[23] Le Bot J,Kirkby E A.Diurnal uptake of nitrate and potassium during the vegetable growth of tomato plants[J].J Plant Nutr,1992,15 (2):247-262.
[24] Lillo C.Light regulation of nitrate uptake,assimilation and metabolism[M] //Amancio S and Stulen I.Plant Ecophysiology Dordrecht:Kluwer Academic Publisher,2004:149-184.
[25] Figueroa F L.Photoregulation of nitrogen metabolism and protein accumulation in the red alga Corallina elongata Elliset Soland[J].Zeitshriftfr Naturforschung,Section CBiosciences,1993,48:788-794.
[26] Lopez-Figueroa F,Ruediger W.Stimulation of nitrate net uptake and reduction by red and blue light and reversion by far-red light in the green alga Ulva-Rigida[J].J Phycology,1991,27:389-394.
[27] Aparicio P J,Quinones M A.Blue light a positive switch signal for nitrate and nitrite uptake by the green alga Monoraphidium braunii[J].Plant Physiol,1991,95:374-378.
[28] Ullrich W R,Lazarova J,Ullrich C L,et al.Nitrate uptake and extracellular alkalinization by the green alga Hydrodictyon reticulum in blue and red light[J].J Exp Bot,1998,49:1157-1162.
[29] Maetschke M,Riedel J,Tischner R.Evidence for signal transduction in the stimulation of nitrate uptake by blue light in Chlorella saccharophila[J].Photochem and Photobiol,1997,66:128-132.
[30] Tischner R.Nitrate uptake and reduction in higher and lower plants[J].Plant Cell and Environment,2000,23:1005-1024.
[31] Delhon P,Gojon A,Tillard P,et al.Diurnal regulation of NO3-uptake in soybean plants.4.Dependence on current photosynthesis and sugar availability tothe roots[J].J Exp Bot,1996,47:893-900.
[32] Cardenas-Navarro R,Adamowicz S,Robin P.Diurnal nitrate uptake in young tomato (Lycopersicon esculentum Mill.) plants:test of a feedback-based model[J].Journal of Experimental Botany,1998,49 (321):721-730.
[33] Scaife A.A pump/leak//buffer model for plant nitrate uptake[J].Plant Soil,1989,114:139-141.
[34] Lejay L,Tillard P,Lepetit M,et al.Molecular and functional regulation of two NO3-uptake systems by N-and Cstatus of Arabidopsis plants[J].Plant J,1999,18:509-519.
[35] Jones R W,Sheard R W.Nitrate reductase activity:phytochrome mediation of induction in etiolated peas[J].Nature,1972,238:221-222.
[36] Hideo S,Yukio Y.Effects of red,far red,and blue light on enhancement of nitrate reductase activity and on nitrate uptake in etiolated rice seedlings[J].Plant Physiol,1979,63:1098-1101.
[37] Appenroth K J,Oelmuller R,Schuster C,et al.Regulation of transcript level and synthesis of nitrate reductase by phytochrome and nitrate in turions of Spirodela polyrhiza (L.) Schleiden[J].Planta,1992,188:587-593.
[38] Pilgrim M L,Caspar T,Quail P H.et al.Circadian and light-regulated expression of nitrate reductase in Arabidopsis[J].Plant Mol Biol,1993,23:349-362.
[39] Sharma A K,Raghuram N,Chandok M R,et al.Investigations on the nature of the phytochrome-induced transmitter for the regulation of nitrate reductase in etiolated leaves of maize[J].J Exp Bot,1994,45:485-490.
[40] Becker T W,Foyer C,Caboche M.Light-regulated expression of the nitrate reductase and nitrite reductase genes in tomato and in the phytochrome-deficient aurea mutant of tomato[J].Planta,1992,188:39-47.
[41] Goud K V,Sharma R.Retention of photoinduction of cytosolic enzymes in aurea mutant of tomato (Lycopersicon esculentum)[J].Plant Physiol,1994,105:643-650.
[42] Appenroth K J,Meco R,Jourdan V,et al.Phytochrome and post-translational regulation of nitrate reductase in higher plants[J].Plant Science,2000,159:51-56.
[43] Lillo C,Provan F,Appenroth K J.Photoreceptors involved in the regulation of nitrate reductase in Spirodela polyrhiza[J].Journal of Photochemistry and Photobiology B:Biology,1998,44:205-210.
[44] Beevers L,Schrader L E,Flesher D,et al.The role of light and nitrate in the induction of nitrate reductase in radish cotyledons and maize seedlings[J].Plant Physiology,1965,40(4):691-698.
[45] Heimer Y M,Filner P.Regulation of the nitrate assimilation path-way in cultured tobacco cells.Ⅲ.The nitrate uptake system[J].Biochim B iophys Acia,1971,230:362-372.
[46] Ferrari T F,Yoder O C,Filner P.Anaerobic nitrite production by plant cells and tissues:evidence for two nitrate pools[J].Plant Physiol,1973,51:423-431.
[47] Aslam M,Oaks A,Huffaker R C.Effect of light and glucose on the induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves[J].Plant Physiol,1976,58:588-591.
[48] Huber S C,Huber J L,Campbell W H,et al.Comparative studies of the light modulation of nitrate reductase and sucrose-phosphate synthase activities in spinach leaves[J].Plant Physiol,1992,100:706-712.
[49] Morcuende R,Krappe A,Hurry V.Sucrose-feeding leads to increased rates of nitrate assimilation,increased rates of α-oxoglutarate synthesis,and increased synthesis of a wide spectrum of amino acids in tobacco leaves[J].Planta,1998,206:394-409.
[50] Kannangara C G,Woolhouse H W.The role of carbon dioxide,light and nitrate in the synthesis and degadation of nitrate reductase in leaves of Perillafruiescens[J].New Phytol,1967,66:553-561.
[51] Atwins C A,Canvin D T.Nitrate,nitrate and ammonia assimilation by leaves:effects of inhibitors[J].Planta,1975,123:41-51.
[52] Travis R L,Key J L.Correlation between polysome level and the ability to induce nitrate reductase in dark-grown corn seedlings[J].Plant Physiol,1971,48:617-620.
[53] Aslam M,Huffaker R C,Travis R L.The interaction of respiration and photosynthesis in induction of nitrate reductase activity[J].Plant Physiol,1973,52:137-141.
[54] Lillo C,Appenroth K J.Light regulation of nitrate reductase in higher plants:which photoreceptors are involved[J].Plant biol,2001,3:455-465.
[55] 刘丽,甘志军,王宪泽.植物氮代谢硝酸还原酶水平调控机制的研究进展[J].西北植物学报,2004,24(7):1355-1361.
[56] Liu W K,Yang Q C.Effects of short-term treatment with various light intensities and hydroponic solutions on nitrate concentration of lettuce.Acta Agriculturae Scandinavica Section B-Soil and Plant Science,2011,iFirst article:1-5.DOI:10.1080/09064710.2011.580-366.
[57] 周晚来,刘文科,闻婧,等.短期连续光照下水培生菜品质指标变化及其关联性分析[J].中国生态农业学报,2011,19 (6):1319-1323.

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