摘要： 旨为筛选能够显著促进菜豆光合作用及豆荚花色苷合成的芸苔素浓度。采用盆栽试验进行，设置有2个紫菜豆品种紫架豆（A1）、紫玉架豆（A2），5个芸苔素浓度B1：CK，B2：0.05 mg/L，B3：0.10 mg/L，B4：0.15 mg/L，B5：0.20 mg/L，共计10个处理，研究不同芸苔素浓度对菜豆光合特性及豆荚花色苷合成的影响。结果表明：菜豆伸蔓期处理A1B2净光合速率最高，与对照产生显著性差异；在菜豆的开花结荚期A1B5蒸腾速率最高，与对照产生显著性差异；处理A1B4的叶绿素含量最高，与对照产生显著性差异；总黄酮含量为处理A2B5最高，且与对照产生显著性差异，含量提高了40.24%；原花青素含量为处理A1B3效果最佳，且与对照差异显著，含量提高了72.4%；花青素含量为处理A2B3效果最佳，且与对照差异显著；花色苷含量为处理A1B3效果最佳，且与对照差异显著，含量提高了90.07%；综上所述，外源喷施芸苔素对于菜豆的净光合速率、蒸腾速率、叶绿素含量产生显著影响，且芸苔素浓度B2、B5效果最佳，促进效果最为明显；外源喷施芸苔素对菜豆豆荚总黄酮、原花青素、花青素、花色苷有显著影响，且芸苔素浓度B3对各品种均有显著影响，为最佳浓度。

关键词: 菜豆, 芸苔素, 光合, 花色苷

Abstract: To screen the concentration of brassinolide which can significantly promote the photosynthesis and the synthesis of anthocyanins in bean pods.The pot experiment was carried out. Two seaweed bean varieties, Zijiadou(A1)and Ziyujiadou(A2), were set with five brass concentrations B1:CK, B2:0.05 mg/L, B3:0.10 mg/L, B4:0.15 mg/L and B5:0.20 mg/L, 10 treatments of were used to study the effects of different concentrations of brassinin on photosynthetic characteristics and anthocyanin synthesis of bean pods.The results showed that the net photosynthetic rate(Pn)and chlorophyll content of kidney bean treated with A1B2 were the best, which were significantly different from the control, and increased by 23%, 21%, respectively.The transpiration rate(Tr)of treatment A1B5 was the most significant, which increased by 59.7% compared with the control. The total flavonoids content of treatment A2B3 was the highest, which was significantly different from that of the control, increasing by 40.24%.The treatment of A1B3 had the best proanthocyanidin content.Compared with the control group, the content increased by 72.4%.The anthocyanin content of A2B3 treatment was the best, and there was a significant difference between A2B3 and the control;The anthocyanin content of A1B3 treatment was the best, and there was a significant difference between A1B3 and the control, and the content increased by 90.07%.In summary, exogenous brassinolide had significant effects on net photosynthetic rate, transpiration rate and chlorophyll content of bean, and brassinolide concentrations B2 and B5 had the best effects, and the promotion effect was the most obvious.Exogenous brassinolide had significant effects on total flavonoids, procyanidins, anthocyanins and anthocyanins in kidney bean pods, and brassinolide concentration B3 had significant effects on all cultivars, is the optimal concentration.

Key words: Bean, Brassinolide, Photosynthetic, Anthocyanins

中图分类号: 

• S643.01