华北农学报 ›› 2011, Vol. 26 ›› Issue (1): 210-214. doi: 10.7668/hbnxb.2011.01.043

所属专题: 油料作物 土壤肥料 生物技术

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转基因大豆对土壤氨氧化细菌的影响

赖欣, 张永生, 赵帅, 杨殿林   

  1. 农业部环境保护科研监测所,农业部转基因生物生态环境安全监督检验测试中心,天津300191
  • 收稿日期:2010-09-20 出版日期:2011-02-28
  • 通讯作者: 杨殿林(1964- ),男,河北香河人,研究员,博士,主要从事农业生态学研究工作
  • 作者简介:赖欣(1981- ),男,重庆人,助理研究员,硕士,主要从事土壤微生物研究工作
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项资金(2008-aepi-03)

Effect of Transgenic Soybean on Amount and Diversity of Ammonia}xidizingBacteria in Rhizospheric Soil

LAI Xin, ZHANG Yong-sheng, ZHAO Shuai, YANG Dian-lin   

  1. Agro-Environmental Protection Institution;
    Eco-safety Supervision, Inspection Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Tianjin 300191, China
  • Received:2010-09-20 Published:2011-02-28

摘要: 采用DGGE-cloning测序技术与定量PCR技术相结合的方法,研究了转基因大豆对土壤中氨氧化细菌群落多样性的影响。定量PCR试验结果表明,相同的生长时期转基因大豆对氨氧化细菌数量没有显著的影响,而与此同时,土壤中的氨氧化细菌的数量呈现出随生长期先增加后减少的趋势;DGGE图谱分析表明,同一生长时期不同大豆土壤中的氨氧化细菌主要条带一致,这表明生长时期的影响明显大于转基因大豆对土壤氨氧化细菌的影响。

关键词: 转基因大豆, 根际土壤, 氨氧化细菌, 多样性, DGGE-cloning, 定量PCR

Abstract: The genetically modified(GM) crops are more and more concerned by public;meanwhile more andmore GM crops are planted in fields globally. Besides the food safety the ecological risks are also gained focus. In or-der to deeply understand the effect of transgenic soybean on diversity of rhizoperic ammoniaxidizing bacteria in soilDGGEloning and quantitative PCR were used. Both the DGGE oning and results showed that seasonal chan-ges were observed throughout the soybean growth stages indicating the impact of crop growth stage overweigh that ofexogenous gene insertion and transgenic soybean has not diversely affect on rhizosphere ammoniaxidizing bacteria a-bundance and community composition in soil.

Key words: Transenic soybean, Rhizospheric soil, Ammoniaxidizin bacteria, Diversity

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引用本文

赖欣, 张永生, 赵帅, 杨殿林. 转基因大豆对土壤氨氧化细菌的影响[J]. 华北农学报, 2011, 26(1): 210-214. doi: 10.7668/hbnxb.2011.01.043.

LAI Xin, ZHANG Yong-sheng, ZHAO Shuai, YANG Dian-lin. Effect of Transgenic Soybean on Amount and Diversity of Ammonia}xidizingBacteria in Rhizospheric Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 210-214. doi: 10.7668/hbnxb.2011.01.043.

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