摘要： 雷公藤生物碱是雷公藤TripterygiumwilfordiiHook中的主要杀虫活性物质。为进一步阐明雷公藤总生物碱的杀虫活性及杀虫作用机理提供线索和依据，研究采用载毒叶片法测定了雷公藤总生物碱对粘虫(Mythimnaseparata(Walker))三龄幼虫的胃毒毒力，观察了粘虫五龄幼虫的中毒症状，并测定了其对不同中毒阶段粘虫五龄幼虫头部乙酰胆碱酯酶(AChE)活性和乙酰胆碱(ACh)含量的影响。结果表明，雷公藤总生物碱对粘虫三龄幼虫48，72，96，120h的胃毒LC50分别为306.39，216.12，157.18，129.92mg/L；试虫中毒后行动迟缓，停止取食，继而软瘫麻痹(即麻醉)，后复苏(或死亡)，复苏试虫可再次取食、麻醉、复苏(或死亡)，如此反复，直止死亡；雷公藤总生物碱对各中毒期试虫AChE无明显的抑制或激活作用；麻醉期试虫体内ACh的相对含量与同期对照无显著差异，复苏期有所下降。
关键词:
雷公藤,
总生物碱,
粘虫,
中毒症状,
乙酰胆碱酯酶,
乙酰胆碱
Abstract: Alkaloid is the main insecticidal composition in Tripterygium wi如rdii Hook a famous insecticidalplant. In order to study its insecticidal activites and mechanism the stomach toxicity of the total alkaloid against larvaeof My"thimua separates Walker was tested their toxicity symptoms were observed and its effects nn the activities ofAChE and the content of ACh of larval heads in different toxicity stages were assayed.
Key words:
Tripteryium wilfordii Hook,
Total alkaloid,
llythimua separates Walker
中图分类号:
周琳. 雷公藤总生物碱对粘虫幼虫的毒力及对乙酰胆碱酯酶和乙酰胆碱的影响[J]. 华北农学报, 2011, 26(1): 215-218. doi: 10.7668/hbnxb.2011.01.044.
ZHOU Lin. Toxicity of MythimnaTotal Alkaloid fromseparata(Walker) and Effectswil ordii Hook against[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 215-218. doi: 10.7668/hbnxb.2011.01.044.