摘要： 离体和活体处理粘虫幼虫围食膜后,通过SDS-PAGE分析其围食膜蛋白条带的变化、观察其形态学特征、生物测定等方法研究了荧光增白剂FB28和棉铃虫颗粒体病毒增效蛋白(En-Ha)对粘虫幼虫围食膜的破坏作用及其对粘虫核型多角体病毒(MsNPV)的增效作用。结果表明,FB28离体处理围食膜能够解离大部分围食膜蛋白,幼虫摄取含有1%FB28的饲料4 h后,围食膜破裂,呈现出蓝色的荧光,高分子量蛋白条带消失。En-Ha离体处理围食膜,能够降解高分子量蛋白,出现高分子量蛋白条带消失,低分子量蛋白条带出现的现象;幼虫摄取含有9μg/mL En-Ha饲料7 h后,围食膜极易断裂,高分子量蛋白条带消失。经过一段时间恢复实验后,FB28和En-Ha处理的幼虫围食膜都能够恢复正常,其破坏作用是短暂的。生物测定结果表明,FB28和En-Ha均能够通过破坏围食膜完整性提高MsNPV的感染率,使LC50显著降低。

关键词: 荧光增白剂, 增效蛋白, 粘虫核型多角体病毒, 围食膜, 增效作用

Abstract: The disruptive effects of Fluorescent Brightener 28( FB28) and Heliothis armigera granulovirus enhancing ( En -Ha) on the peritrophic membrane( PM) of larvae and enhancing effectsonMsNPV in Mythima separata were studied through SDS -PAGE, structural observation and bioassay.The results indicated that proteins could be dissociated from the PM in vitro by FB28.After the larvae were reared with diets containing 1% FB28 for 4 h, bluish fluorescence appeared on the disrupted PM.SDS -PAGE of PM treatedwith FB28 in vivo showed that some proteins with high molecular weight disappeared.After the PM was treated with En -Ha in vitro, some proteins with high molecular weight disappeared and some with lower molecular weight appeared, which indicated En -Ha could degrade the high -molecular -weight proteins from the PM.The PM became fragile after the larvaewere rearedwith diets containing 9μg/mL En -Ha for 7h, and SDS -PAGE showed that some proteinswith high molecular weight disappeared similar to the profile treated by FB28?However, the ex - periments testified that the disruptive effect could be restored after rearing the larvae on the diet without FB28 and En - Ha.The bioassay showed that FB28 and En -Ha could enhance the vitality of MsNPV and significantly decreased LC 50 by degrading the integrity of PM.

Key words: Fluorescent Brightener 28, Enhancin, MsNPV, Peritrophic membrane, Enhancing effect

中图分类号: 

• S432.4+1