小麦与叶锈菌互作前后基因组甲基化模式分析

doi:10.7668/hbnxb.2008.04.007

华北农学报 ›› 2008, Vol. 23 ›› Issue (4): 28-31. doi: 10.7668/hbnxb.2008.04.007

所属专题： 小麦；生物技术；

小麦与叶锈菌互作前后基因组甲基化模式分析

付胜杰¹, 王晖², 冯丽娜¹, 李星¹, 王珅¹, 杨文香¹, 刘大群¹

1. 河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,河北保定 071001;
2. 济宁医学院,山东济宁 272013

收稿日期:2008-02-25 出版日期:2008-08-28
通讯作者: 杨文香(1966-),女,河北沧州人,博士,教授.博士生导师,主要从事小麦锈病研究;刘大群(1958-),男,河北石家庄人,博上,教授,博士生导师,主要从事植物病害生物防治与分子植物病理学研究
作者简介:付胜杰(1978-),男,山东济宁人,硕士,主要从事小麦锈病研究
基金资助:
国家“973”专项(2005CCA01600);国家“十一五”支撑计划(2006BAD08A05)

Analysis of DNA Methyiation Related on the Interaction between Wheat and Wheat Leaf Rust

FU Sheng-jie¹, WANG Hui², FENG Li-na¹, LI Xing¹, WANG Shen¹, YANG Wen-xiang¹, LIU Da-qun¹

1. Department of Plant Pathology,College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China;
2. Jining Medical College,Jining 272013,China

Received:2008-02-25 Published:2008-08-28

摘要/Abstract

摘要： 首次使用甲基化敏感扩增多态性(MSAP)技术分析两种小麦材料近等基因系TcLr19及其感病亲本Thatcher的甲基化水平,同时比较了苗期接种叶锈菌生理品种THTT前后基因组DNA胞嘧啶甲基化模式。60对选扩引物对接种前后的小麦DNA进行全基因组筛选,共得到3 554个片段。其中998个片段是两种甲基化模式中的一种,小麦近等基因系TcLr19及其感病亲本Thatcher的甲基化水平约为28.1%。在所有的引物中,并没有直接分离得到接菌前后的甲基化模式的差异。结果初步表明,叶锈菌可能并没有诱导植物基因组DNA胞嘧啶位点的甲基化模式变化。

关键词: DNA甲基化, 小麦叶锈菌, 甲基化敏感扩增多态性, 抗病性

Abstract: DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. The wheat near-isogenic line TcLr19 and Thatcher is race-specific resistance and compatible in the interaction with Puccinia triticina respectively. Using methylation -sensitive amplified polymorphism( MSAP)analysis,we compared the patterns of cy- tosine methylation in TcLr19 and Thatcher that had been inoculated with Puccinia triticina,subjected to left untreated. In all,3 554 DNA fragments,each representing a recognition site cleaved by either or both of two isoschizomers,were amplified using 60 pairs of selective primers. A total of 998 sites were found to be one of the methylated. The results suggest that no differences in cytosine methylation exist between seedlings inoculated and those unchallenged.

Key words: DNA methylation, Puccinia triticina, MSAP, Resistance

中图分类号:

S512.01

付胜杰, 王晖, 冯丽娜, 李星, 王珅, 杨文香, 刘大群. 小麦与叶锈菌互作前后基因组甲基化模式分析[J]. 华北农学报, 2008, 23(4): 28-31. doi: 10.7668/hbnxb.2008.04.007.

FU Sheng-jie, WANG Hui, FENG Li-na, LI Xing, WANG Shen, YANG Wen-xiang, LIU Da-qun. Analysis of DNA Methyiation Related on the Interaction between Wheat and Wheat Leaf Rust[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 28-31. doi: 10.7668/hbnxb.2008.04.007.

http://www.hbnxb.net/CN/Y2008/V23/I4/28

参考文献

[1] Jost J P,Saluz H P.DNA methylation:molecular biology and biologicai signicance[M].Cance Springer-Verlag,Basel,1993.
[2] Finnegan E J,Peacock W J,Dennis E S.DNA methylation.a key regulator of plant development and other processes[J].Curropin Genet Dev,2000,10:217-223.
[3] Haz I A,Anjum M A,Grewal A G,et al.DNA methyrlaton-an essential mechanism in plant molecular biology[J].Acm physiol Plant,200l,23:491-499.
[4] Martienssen R A.Coiot V.DNA methylafion and epigenetic inheritance in plants and lamentous fungi[J].Science,2001,293:1070-1074.
[5] Paszkowski J,Whitham S A.Gene silencing and DNA methylation processes[J].Curr Opin Plant Biol,2001,4:123-129.
[6] Wassenegger M.RNA-directed DNA methylation[J].Plant Mol Biol,2000,43:203-220.
[7] Xu M L,LI X Q,Korban S S.AFLP-hased detection of DNA methytation[J].PIant Mol Biol Rep,2000,18:361-368.
[8] Reyna-Lopez G E,Simpson J,Ruiz-Herrera J.Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphisms[J].Mol Gen Genet,1997,253:703 710.
[9] Madlung A,Mnsueili R W,Watson B,et al.Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids[J].Plant Physiol,2002,129:733 746.
[10] Xiong L Z,Xu C G,Saghai Maroof M A,et al.Patterns of cytosine methylation in all elite rice hybrid and its parental lines,detected by a methylation-sensitive amplification polymorphism technique[J].Mol Gen Genet,1999,26l:439-446.
[11] Shaked H,Kashkush K,Ozkan H,et al.Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat[J].Plant Cell,2001,13:1749-1759.
[12] Sheranm J D,Talbert L E.Vernalization-induced changes of the DNA methylation pattern in winter wheat[J].Genome 2002,45:253-260.
[13] Sha A H,Lin X H,Huang J B,et al,Analysis of DNA methvlation related to rice adult plant resistance to bacterial blight based on methylation-sensitive AFLP(MSAP)analysis[J].Mol Gen Genomies,2005,273:484-490.
[14] 贾继增.小麦的抗叶锈基因及抗原[J].国外农学-麦类作物,1990(2):32-34.
[15] Denissen C J M.Innuence of race and post infection temperature on two components of partial resistance to wheat leaf in seedings ofwheat[Jj.Euphytica,199l,58(1):13-20.
[16] Komer J A.Genetics of resistance to wheat leaf rust[J].ARIIR Rev Phytopathol,1996,34:435-455.
[17] 白志英,王冬梅,侯春燕.等.小麦叶锈菌侵染过程的显微和超微结构[J].细胞生物学杂志,2003,25(6):393-397.
[18] 黄国红,康振生,朱之育,等.小麦叶锈菌在感病寄主上发育的组织病理学和超微结构研究[J].植物病理学报,2003,33(1):52-56.
[19] 黄围红,王瑶.康振生,等.小麦品种及单基因系与叶锈菌互作的组织学和超微结构研究[J].西北农林科技大学学报:自然科学版,2003,31(2):45-49.
[20] 杨文香.Lr37、Lr44 AFLP分子标记及124个小麦品种(系)抗叶锈基因鉴定[D].保定:河北农业大学,2003.
[21] Burn J E,Smyth D R,Peacock W J,et al.Genes confemng late flowering in Arabidopsis thaliana[J].Genetica,1993,90:147-155.
[22] Finnegan E J,Genger R K,Kovac K,et al.DNA methylation and the promotion of flowering by vernalization[J].Proe Natl Acad Sci,1998,95:5824-5829.

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