高压静电场对羽衣甘蓝自交不亲和系种子基因组DNA甲基化的影响

doi:10.7668/hbnxb.2015.S1.002

华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 8-18. doi: 10.7668/hbnxb.2015.S1.002

所属专题： 生物技术；

高压静电场对羽衣甘蓝自交不亲和系种子基因组DNA甲基化的影响

张旸¹, 胡中影¹, 李娜², 丁兵¹, 王江¹, 解莉楠¹

1. 东北林业大学生命科学学院, 黑龙江哈尔滨 150040;
2. 黑龙江生物科技职业学院, 黑龙江哈尔滨 150025

收稿日期:2015-10-24 出版日期:2015-12-28
通讯作者: 解莉楠(1978-),女,黑龙江哈尔滨人,副教授,博士,主要从事植物逆境胁迫研究.
作者简介:张旸(1977-),女,河北石家庄人,副教授,博士,主要从事植物发育生物学研究.
基金资助:
中央高校基本科研业务费专项资金项目(2013BAQ01);哈尔滨市科技创新人才研究专项资金项目(青年科技创新人才)(2013RFQXJ036)

Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala

ZHANG Yang¹, HU Zhong-ying¹, LI Na², DING Bing¹, WANG Jiang¹, XIE Li-nan¹

1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China;
2. Heilongjiang Vocational College of Biology and Technology, Harbin 150025, China

Received:2015-10-24 Published:2015-12-28

摘要/Abstract

摘要： DNA甲基化在植物的生长发育中具有重要的调控作用,它是植物正常生长发育所必需的.采用甲基化敏感扩增多态性(Methylation sensitive amplification polymorphism,MSAP)技术,以羽衣甘蓝自交不亲和系9^#种子为研究对象,从表观遗传学的角度,对其生长发育过程以及高压静电场处理条件下基因组DNA甲基化水平及模式变化情况进行研究,探讨自交不亲和系种子退化现象与表观遗传规律的相关性.结果表明,自交不亲和系9^#种子经高压静电场处理后,同时发生甲基化与去甲基化事件,以去甲基化为主.其中全甲基化水平略有升高,半甲基化水平下降明显,总甲基化水平下降;通过记录和计算种子在萌发过程中的各项指标,结果表明,高压静电场可促进9^#种子的萌发和幼苗的生长发育,改善种子品质,表明高压静电场处理是恢复或延迟羽衣甘蓝自交不亲和系退化种子生长势,保持自交后代品质的有效手段之一.

关键词: 羽衣甘蓝, 自交不亲和性, DNA甲基化, 高压静电场

Abstract: DNA methylation plays an important role in regulating plant growth,and it is necessary for plant normal growth and development.In this experiment,methylation sensitive amplification polymorphism (MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incompatible line 9^# and the change of DNA methylation status in 9^# after high-voltage electric field.The correlation was discussed between seed degradation phenomena of self-incompatible line and epigenetic one.The results were shown as follows:After high-voltage electric field treatment,both methylation and demethylation occurred;and demethylation seemed to be predominant,the proportion of full-methylation increased slightly and semi-methylation declined clearly and brought about the result of total methylation declined;the physiological indexes of 9^# in seed germination,which showed high-voltage electric field,could promote seed germination and seeding growth and improve the quality of seeds.High-voltage electric field was an effective means to delay and restore growth vigor,and to maintain the quality of self-crossed progeny.

Key words: Brassica oleracea var.acephala, Self-incompatibility, DNA methylation, High-voltage electric field

中图分类号:

Q78
S635.03

张旸, 胡中影, 李娜, 丁兵, 王江, 解莉楠. 高压静电场对羽衣甘蓝自交不亲和系种子基因组DNA甲基化的影响[J]. 华北农学报, 2015, 30(S1): 8-18. doi: 10.7668/hbnxb.2015.S1.002.

ZHANG Yang, HU Zhong-ying, LI Na, DING Bing, WANG Jiang, XIE Li-nan. Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 8-18. doi: 10.7668/hbnxb.2015.S1.002.

http://www.hbnxb.net/CN/Y2015/V30/IS1/8

参考文献

[1] 陈静,单成伟.羽衣甘蓝日光温室栽培技术[J].吉林蔬菜,2008,01(1):40-41.
[2] 孟金陵.植物生殖遗传学[M].北京:科学,1995.
[3] Dixit R,Nasrallah J B.Recognizing self in the self-incompatibility response[J].Plant Physiology,2001,125(1):105-108.
[4] Zhang Y,Zhao Z,Xue Y.Roles of proteolysis in plant self-incompatibility[J].Annual Review of Plant Biology,2009,60:21-42.
[5] 寇小培.分子标记辅助选育甘蓝型油菜自交不亲和系[D].武汉:华中农业大学,2012.
[6] 于晓敏.羽衣甘蓝SRK和SCR的克隆表达及克服自交不亲和性的研究[D].哈尔滨:东北林业大学,2007.
[7] Cramariuc R,Donescu V,Popa M,et al.The biological effect of the electrical field treatment on the potato seed:agronomic evaluation[J].Journal of Electrostatics,2005,63(6/10):837-846.
[8] 李娜,张旸,解莉楠,等.植物DNA甲基化研究进展[J].植物生理学报,2012,48(11):1027-1036.
[9] Sulewska A,Niklinska W,Kozlowski M,et al.DNA methylation in states of cell physiology and pathology[J].Folia histochemica et cytobiologica/Polish Academy of Sciences,Polish Histochemical and Cytochemical Society,2007,45(3):149-158.
[10] Jullien P E,Susaki D,Yelagandula R,et al.DNA methylation dynamics during sexual reproduction in Arabidopsis thaliana[J].Current Biology,2012,22(19):1825-1830.
[11] Li X,Wang X,He K,et al.High-resolution mapping of epigenetic modifications of the rice genome uncovers interplay between DNA methylation,histone methylation,and gene expression[J].The Plant Cell,2008,20(2):259-276.
[12] Berger F,Chaudhury A.Parental memories shape seeds[J].Trends in Plant Science,2009,14(10):550-556.
[13] Tantikanjana T,Nasrallah M E,Nasrallah J B.Complex networks of self-incompatibility signaling in the Brassicaceae[J].Current Opinion in Plant Biology,2010,13(5):520-526.
[14] 张本华,李成华.高压电场对大豆种子活力影响的试验研究[J].沈阳农业大学学报,2006,37(4):660-662.
[15] Han Z,Fumio H.Efieet of high electric fields on the germ ination and early growth of some vegetable seeds[J].Journal of the Japanese Society for Horticultural Science,1997,66(2):347-352.
[16] 许月英,征荣,杨体强,等.不同强度电场对雀麦种子萌发及幼苗生长的影响[J].内蒙古大学学报:自然科学版,2003,34(3):294-297.
[17] Liu B,Brubaker C L,Mergeai G,et al.Polyploid formation in cotton is not accompanied by rapid genomic changes[J].Genome,2001,44(3):321-330.
[18] 胡适宜.被子植物生殖生物学[M].北京:高等教育出版社,2005:134-176.
[19] 汤青林,宋明,王小佳.芸苔属植物自交不亲和性及其机理研究进展[J].生物工程进展,2001,21(4):22-25,10.
[20] 陆光远,伍晓明,陈碧云,等.油菜种子萌发过程中DNA甲基化的MSAP分析[J].科学通报,2005,50(24):2750-2756.
[21] 于爱真,蔡兴旺,李明,等.高压静电场分离水稻、油菜及芝麻种子对萌发期生物效应的影响[J].农业工程学报,1995,12(4):139-143.
[22] 杨金兰,柳李旺,龚义勤,等.镉胁迫下萝卜基因组DNA甲基化敏感扩增多态性分析[J].植物生理与分子生物学学报,2007,33(3):219-226.
[23] Fisk D G,Walker M B,Barkan A.Molecular cloning of the maize gene crp1 reveals similarity between regulators of mitochondrial and chloroplast gene expression[J].The EMBO Journal,1999,18(9):2621-2630.
[24] Cushing D A,Forsthoefel N R,Gestaut D R,et al.Arabidopsis emb175 and other ppr knockout mutants reveal essential roles for pentatricopeptide repeat (PPR) proteins in plant embryogenesis[J].Planta,2005,221(3):424-436.
[25] Rivals E,Bruyère C,Toffano-Nioche C,et al.Formation of the Arabidopsis pentatricopeptide repeat family[J].Plant Physiology,2006,141(3):825-839.
[26] 范敏,金黎平,刘庆昌,等.马铃薯PPR蛋白家族基因SoDIPPR的克隆及其在干旱条件下的表达特征分析[J].中国农业科学,2008,41(8):2249-2257.
[27] Srinivasula S M,Hegde R,Saleh A,et al.A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis[J].Nature,2001,410(6824):112-116.
[28] 姜立杰,曹家树.芸薹属植物自交不亲和性的分子机制[J].植物学通报,2001,18(4):411-417.
[29] Nasrallah M E,Wallace D H.Immunogenetics of self-incompatibility in Brassica oleracea[J].Heredity,1967,22:519-527.
[30] 李晓波,张俊武.真核生物中锌指蛋白的结构与功能[J].中国生物化学与分子生物学报,2009,25(3):206-211.
[31] Lovering R,Hanson I M,Borden K L,et al.Identification and preliminary characterization of a protein motif related to the zinc finger[J].Proc Natl Acad Sci USA,1993,90(6):2112-2116.
[32] Peng H,Begg G E,Schultz D C,et al.Reconstitution of the KRAB-KAP-1 repressor complex:a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions[J].Journal of Molecular Biology,2000,295(5):1139-1162.
[33] Cohen N,Sharma M,Kentsis A,et al.PML RING suppresses oncogenic transformation by reducing the affinity of eIF4E for mRNA[J].The EMBO journal,2001,20(16):4547-4559.
[34] Buchwald G,Van der stoop P,Weichenrieder O,et al.Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b[J].EMBO J,2006,25(11):2465-2474.

选择文件类型/文献管理软件名称

选择包含的内容

高压静电场对羽衣甘蓝自交不亲和系种子基因组DNA甲基化的影响

Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	严婷, 李霞, 曹悦, 吴博晗, 王净, 张嫚嫚. *5-氮杂胞苷对高表达转玉米C₄-PEPC基因水稻耐旱性的影响*[J]. 华北农学报, 2021, 36(4): 96-107.
[2]	杨勤, 柴志欣, 王吉坤, 王会, 钟金城. *牦牛ACTA1基因启动子区克隆、DNA甲基化与组织表达相关性分析*[J]. 华北农学报, 2020, 35(1): 222-229.
[3]	何平, 疏冕, 蔡晓丹, 傅雪琳. 栽培稻×药用野生稻种间杂种基因组DNA甲基化的遗传与变异研究[J]. 华北农学报, 2017, 32(4): 19-31.
[4]	张亚玲, 幸宏伟, 郭宁, 韩硕, 宗梅, 刘凡, 王桂香. 羽衣甘蓝抗寒生理特性研究[J]. 华北农学报, 2016, 31(4): 168-176.
[5]	徐志军, 黄莉, 姜慧芳. 青枯菌诱导下花生基因组DNA表观遗传变化的MSAP分析[J]. 华北农学报, 2015, 30(2): 93-99.
[6]	付胜杰, 王晖, 冯丽娜, 李星, 王珅, 杨文香, 刘大群. 小麦与叶锈菌互作前后基因组甲基化模式分析[J]. 华北农学报, 2008, 23(4): 28-31.
[7]	杨锐, 余阳俊, 徐家炳, 陈广, 张凤兰, 孙继志, 孙天水. 花期喷盐水结合蜜蜂授粉克服大白菜自交不亲和性试验[J]. 华北农学报, 1995, 10(2): 77-81.
[8]	王怀名, 杜广岑, 贾翠莹, 谭学文, 王贤. 观赏羽衣甘蓝的离体繁殖[J]. 华北农学报, 1995, 10(1): 64-69.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

高压静电场对羽衣甘蓝自交不亲和系种子基因组DNA甲基化的影响

Effects of High-voltage Electric Field on DNA Methylation of Self-incompatibility Seed of Brassica oleracea var.acephala

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价