青枯菌诱导下花生基因组DNA表观遗传变化的MSAP分析

doi:10.7668/hbnxb.2015.02.017

摘要/Abstract

摘要： 以高感青枯病品种中花12号和高抗青枯病品种远杂9102为材料,调查青枯菌接种12 h后花生基因组DNA的甲基化水平和变化模式。甲基化敏感扩增多态性(Methylation-sensitive amplification polymorphism,MSAP)分析表明,正常生长的中花12号和远杂9102的基因组DNA甲基化比率均为35.1%,经青枯菌接种12 h后,中花12号降低到31.3%,而远杂9102的基因组DNA甲基化比率则升高至37.2%。与对照相比,青枯菌接种12 h胁迫下中花12号和远杂9102基因组DNA的甲基化和去甲基化分别为5.9%,12.4%,18.3%,11.9%。由此推测,高抗青枯菌的花生经青枯菌侵染后基因组DNA某些位点的甲基化状态发生变化而使基因表达发生了改变,产生或启动对青枯菌胁迫的能动应激机制。

关键词: 花生, 青枯菌, DNA甲基化, 甲基化敏感扩增多态性

Abstract: A high sensitive variety Zhonghua 12 and a high resistance variety Yuanza 9102, infected by R.solanacearum, were used to assess the level and changing pattern of DNA methylation by methylation-sensitive amplified polymorphism (MSAP) technique.The results showed that the level of global DNA methylation of the two varieties were all 35.1% under normal condition and after infection 12 hours, the level of Zhonghua 12 decreased to 31.3% while the level of Yuanza 9102 increased to 37.2%.Methylation and demethylation of DNA of the two varieties were 5.9%, 12.4%, 18.3% and 11.9% after infection.It indicated that the genomic DNA methylation status of the highly resistance variety of peanut were changed in some site after infection by R.solanacearum and changed the expression of some genes for initiating stress mechanism.

Key words: Peanut, R.solanacearum, DNA methylation, MASP

中图分类号:

S565.2

徐志军, 黄莉, 姜慧芳. 青枯菌诱导下花生基因组DNA表观遗传变化的MSAP分析[J]. 华北农学报, 2015, 30(2): 93-99. doi: 10.7668/hbnxb.2015.02.017.

XU Zhi-jun, HUANG Li, JIANG Hui-fang. MSAP Analysis of Epigenetic in Peanut under Ralstonia solanacearum Induced[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 93-99. doi: 10.7668/hbnxb.2015.02.017.

http://www.hbnxb.net/CN/Y2015/V30/I2/93

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