摘要： 为了给河北地区预防和控制猪繁殖与呼吸综合征(PRRS)提供理论数据,根据GenBank公布的PRRSV CH-1a株和JXA1株的核苷酸序列,设计并合成三对特异性引物。应用RT-PCR方法分别扩增PRRSV HB-4(hs)株的ORF5,ORF6,ORF7基因片段。分别将片段克隆入pGM-T载体后进行测序,并与多株GenBank中发表的PRRSV毒株ORF5-7基因的核苷酸序列进行比较和变异分析。结果表明,PRRSV HB-4(hs)株属于美洲型,其与近两年流行的毒株群相似性高达98.8%-99.5%;与早些年流行毒株群相似性较低,为91.9%~92.6%。系统进化树表明:HB-4(hs)株及近两年流行的毒株可能均由早期分离的河北HB-1(sh)株进化而来。

关键词: 猪繁殖与呼吸综合征病毒, ORF5-7基因, 遗传变异分析

Abstract: In order to offer the theoretical data for prevent and control PRRS that unparalleled outbreak in Hebei. According to the reported complete nucleotide sequence of PRRSV CH-1a and JXA1 inGeneBank,3 pairsof primers were designed to amplify ORF5-7 gene of HB -4( hs)strain with Primer 5. 0 software. The ORF5-7 gene nucleotide se- quenceswere obtained by RT-PCR and sequenced after being cloned directly into the pGM-T vector. The obtained sequences were analyzed and compared with the published complete nucleotide sequences of other strains in GeneBank by DNAstar software. The results showed that the PRRSV HB-4( hs)strainwas clustered within North American genotopy. The ORF5-7 gene of HB- 4( hs)strain showed highest homology of 98. 8%- 99. 5%to the strains were isolated in recent years,showed 91. 9%- 92. 6%identity with the strainswere isolated in last years. The phylogenetic trees revealed the strains were isolat- ed in recent years had close relationshipwith HB -1( sh)strain.

Key words: Porcine reproductive and respiratory syndrome virus, ORF5-7 gene, Heredity and variation analysis

中图分类号: 

• S432.4+1