摘要： 为了解PRRSV Nsp2基因缺失变异株的致病性,采用2个Nsp2基因分别连续缺失3个和89个碱基的PRRSV变异株Hn-2(GenBank:FJ23719)和Hn-(GenBank:FJ23721)的病毒液,人工滴鼻感染断奶仔猪,同时用Marc-15健康仔猪细胞培养液滴鼻作对照,在感染后0,7,1,2,32,1,50,60和70 d无菌采血,分离血清,用RTPCR方法检测病毒、用N-ELISA方法和免疫荧光抑制试验分别检测抗PRRSV N蛋白抗体和抗PRRSV中和抗体,比较分析不同时期病毒复制和抗体产生的变化规律.结果表明,仔猪感染Hn-2和Hn-毒株后7～1 d均能检测到病毒,第7天能检测到抗PRRSV N蛋白抗体,并一直维持较高水平,第1和50天检测到较低水平的中和抗体,随后中和抗体效价逐渐升高.试验猪均未出现死亡,表明PRRSV Nsp2基因缺失变异株不一定导致感染猪的死亡.
关键词:
猪繁殖与呼吸综合征病毒,
N蛋白,
中和抗体,
Nsp2基因
Abstract: In order to understand the pathogenicity of PRRSV about Nsp2 gene deletion mutant,The PRRS negative healthy piglets of 20 days-old were inoculated by intranasal with a Mutant of PRRSV Hn-2(GenBank:FJ237419)and Hn-4(GenBank:FJ237421)strains respectively,which delete 3 base pairs and 89 base pairs in the Nsp2 gene respectively,while healthy piglets were used for control,the sera were separated aseptically on the 0,7,14,24,32,41,50,60,70 d respectively,using RT-PCR to detect the virus,using ELISA method and immunofluorescence inhibition test for detection of anti-PRRSV N protein antibody and neutralizing antibodies,variation of the virus and antibody were comparied in different periods of time.The results showed that the PRRSV could be detected in 7-14 d after infected with the Mutant Hn-2,and Hn-4 strain,anti-PRRSV N protein antibodies could be detected on the 7th day and has maintained a high level,respectively,the low level of neutralizing antibodies could be detected respectively on 41 d and 50 d,and the neutralization antibody increased gradually.The test pigs were not of death,indicating that the mutant PRRSV with deleted Nsp2 gene does not necessarily lead to pig death.
Key words:
PRRSV,
N protein,
neutralization antibody,
Nsp2 gene
中图分类号:
张凤华, 卢晓艳, 徐红运, 赵琳, 刘玉松, 范旭, 夏平安, 崔保安. 感染PRRSV Nsp2基因部分缺失变异株的仔猪抗体变化规律[J]. 华北农学报, 2011, 26(3): 68-71. doi: 10.7668/hbnxb.2011.03.015.
ZHANG Feng-hua, LU Xiao-yan, XU Hong-yun, ZHAO Lin, LIU Yu-song, FAN Xu, XIA Ping-an, CUI Bao-an. The Changed Rule of Infected Piglets by a Mutant of Porcine Reproductive and Respiratory Syndrome Virus with Nsp2 Gene Deletion[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 68-71. doi: 10.7668/hbnxb.2011.03.015.