摘要： 采用RT-PCR方法,分段扩增出猪繁殖与呼吸综合征病毒(PRRSV)Shanxi-6株15个基因片段,并将这15个片段分别连接到pMD18-T载体上进行克隆测序、拼接后,得到PRRSV Shanxi-6株全基因组长15 320 bp(不包含PolyA尾),包含9个开放阅读框,5’UTR长189 bp,3’UTR长150 bp。基因组序列分析结果表明,Shanxi-6株与欧洲型代表毒株LV的核苷酸序列同源性为54.35%,与美洲型代表毒株VR-2332的核苷酸序列同源性为88.80%,与HPPRRSV毒株(JXA1、HUN4、HuN、HUB1)亲缘关系最近,核苷酸序列同源性高达97.44%～97.74%,且在非结构蛋白Nsp2上有30个氨基酸的缺失。
关键词:
猪繁殖与呼吸综合征病毒,
基因组,
序列分析
Abstract: The fifteen gene fragments of Shanxi-6 strain of porcine reproductive and respiratory syndrome were amplified respectively by RT-PCR, cloned into pMD18-T vector and sequenced. The genome of Shanxi-6 strain in length was 15 320 bp excluding PloyA tail, containing 9 open reading frames (ORFs), the 5'UTR in 189 bp and the 3'UTR in 150 bp. The complete genomic analysis showed that Shanxi-6 strain shared 54. 35% of nucleotide ho- mology with European genotype (LV) and shared 88. 80% homology with American genotype (VR-2332) . Shanxi- 6 strain has correlative genetic relationship and shared 97. 44%-97. 74% homology with highly pathogenic PRRSV (JXA1, HUN4, HuN, HUB1) . 30 amino acids deletion was exhibited in Nsp2 protein of Shanxi-6 strain.
Key words:
PRRSV,
Complete genome,
Sequence analysis
中图分类号:
韩一超, 刘文俊, 姚敬明, 吴忻, 王娟萍, 武守艳, 陈剑波, 孟帆, 郭慧慧, 杨丽华, 雷宇平, 樊振华. 猪繁殖与呼吸综合征病毒Shanxi-6株全基因序列分析[J]. 华北农学报, 2014, 29(2): 62-65. doi: 10.7668/hbnxb.2014.02.011.
HAN Yi-chao, LIU Wen-jun, YAO Jing-ming, WU Xin, WANG Juan-ping, WU Shou-yan, CHEN Jian-bo, MENG Fan, GUO Hui-hui, YANG Li-hua, LEI Yu-ping, FAN Zhen-hua. Sequence Analysis of Whole Genome of Porcine Reproductive and Respiratory Syndrome Virus Shanxi-6 Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 62-65. doi: 10.7668/hbnxb.2014.02.011.