哥斯达黎加链霉菌A-m1的促生作用及解析

doi:10.7668/hbnxb.20192035

摘要/Abstract

摘要： 为了探究哥斯达黎加链霉菌菌株A-m1对植物的潜在促生作用及机制，首先利用番茄苗生长分析菌株A-m1具有的促生作用，然后对菌株A-m1的全基因组序列进行比对分析，解析促生作用相关的遗传基础，最后使用沙尔科夫斯基反应法（Salkowski）测定菌株A-m1产吲哚乙酸（IAA）能力，铬天青（CAS）法验证产铁载体活性。结果表明，菌株A-m1对番茄幼苗表现出显著促生作用，促生作用与菌体添加量呈正相关，菌株固体发酵产物在基质中添加量为40 g/kg时，番茄植株的叶长、株高、整株鲜质量、整株干质量、根干质量和茎秆直径分别增加了107.1%，70.7%，439.9%，427.4%，473.4%和78.7%。菌株A-m1基因组包含有促进植物生长相关的生长素、细胞分裂素、铁载体、ACC脱氨酶、植酸酶、磷酸酶、固氮酶基因；后续的生化分析进一步证明，菌株A-m1具有合成IAA和铁载体的能力。番茄幼苗的生长证实了菌株A-m1的促生作用，基因组分析和生化验证解析了促生的机制，为该菌株的进一步开发利用奠定了基础。

关键词: 哥斯达黎加链霉菌, 促生作用, 基因组, IAA, 铁载体

Abstract: The growth promoting effect and mechanism of Streptomyces costaricanus strain A-m1 on tomato seedlings were analyzed. At first, the experiment clarified the effect of adding the solid fermentation product of strain A-m1 to the substrate on the growth of tomato seedlings. Then, the whole genome sequences of strain A-m1 were compared to analyze the genetic basis related to growth promotion. Finally, the ability of strain A-m1 to produce indoleacetic acid(IAA) and siderophore was measured by Salkowski method and chromazurol(CAS) method. The results showed that the strain A-m1 significantly promoted the growth of tomato seedlings. The growth-promoting effect was positively related to the amount of Streptomyces costaricanus added. When the content of the solid fermentation product in the substrate was 40 g/kg, the leaf length, plant height, whole plant fresh quality, whole plant dry quality, root dry quality and stem diameter of tomato plants increased by 107.1%, 70.7%, 439.9%, 427.4%, 473.4% and 78.7%, respectively. Genome analysis showed that the strain A-m1 had the genes related to auxin, cytokinin, siderophore, ACC deaminase, phytase, phosphatase and nitrogenase, which could promote plant growth;Biochemical analysis of the strain A-m1 further validated the ability to synthesize IAA and siderophore. Growth of tomato seedling confirmed the promoting effect of the strain A-m1, and genome analysis and biochemical verification further clarified the growth-promoting mechanism of the strain, which laid the foundation for further development and utilization of the strain.

Key words: Streptomyces costaricanus, Growth promoting effect, Genome, IAA, Siderophore

中图分类号:

S476.1

史娟, 李永丽, 常乐, 郑炜凡, 周洲, 曲良建. 哥斯达黎加链霉菌A-m1的促生作用及解析[J]. 华北农学报, 2021, 36(4): 205-211. doi: 10.7668/hbnxb.20192035.

SHI juan, LI Yongli, CHANG Le, ZHENG Weifan, ZHOU Zhou, QU Liangjian. Analysis of the Growth Promoting Effect and Mechanism of Streptomyces costaricanus A-m1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 205-211. doi: 10.7668/hbnxb.20192035.

http://www.hbnxb.net/CN/Y2021/V36/I4/205

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