摘要： 稻瘟病是我国水稻主产区的第一大病害。Pi-b基因在我国很多稻区都表现出广谱抗病性并得到了应用。然而该基因在黑龙江省的分布及应用未见报道。本研究利用源于Pi-b基因本身的特异性分子标记,结合黑龙江省稻瘟病菌混合接种鉴定及田间自然抗病鉴定,检测和分析了来自黑龙江省农垦科学院水稻所36份水稻品种Pi-b基因的分布情况。结果表明,垦稻06-193、垦稻06-774、垦稻19、垦稻13这4份材料含有Pi-b基因。该结论有助于含有Pi-b基因的品种合理布局及利用,为进一步进行抗病基因聚合育种打下了基础。

关键词: 水稻, 稻瘟病, 抗性基因, 显性标记

Abstract: The major blast resistance genes Pi-b,showed good resistance spectrum to rice blast in many rice growing areas of China,and has been utilized in rice breeding and production. However,the distribution of this gene in rice of Heilongjiang Province has not been well investigated,resulting in the blindness of their utilization in rice breeding. In this study,the existences of the gene in 36 rice cultivars /lines were rapidly determined using the dominant DNA marker Pi-b /Pi-b alleles. The Pi-b gene alone were found in four cultivars /lines. i. e. Kendao 06-193,Kendao06-774,Kendao19,Kendao13. These results are useful for rational utilization and layout of the rice cultivars with the Pi-b gene in cold region,and for incorporating and stacking this gene into elite present leading cultivars / lines by marker assisted selection in rice breeding.

Key words: Rice, Rice blast, Resistance gene, Dominant DNA marker

中图分类号: 

• Q78