华北农学报 ›› 2010, Vol. 25 ›› Issue (2): 160-163. doi: 10.7668/hbnxb.2010.02.032

所属专题: 小麦 生物技术

• 论文 • 上一篇    下一篇

不同基因型小麦品种灌浆期籽粒库活性的差异

王文静1, 刘福建2   

  1. 1. 郑州牧业工程高等专科学校 生物工程系, 河南 郑州 450011;
    2. 商丘技师学院, 河南 商丘 476100
  • 收稿日期:2009-12-26 出版日期:2010-04-28
  • 作者简介:王文静(1970-),女,河南新乡人,副教授,硕士,主要从事生理生化教学和科研工作.
  • 基金资助:
    河南省教育厅自然科学研究项目(2008A208021)

Studies on the Differences of the Sink Activity in the Kernel During Grain Filling of the Different Genotype Winter Wheat

WANG Wen-jing1, LIU Fu-jian2   

  1. 1. Department of Biology Engineering, Zhengzhou Animal Husbandry Engineering College, Zhengzhou 450011, China;
    2. Shangqiu Technician Institution, Shangqiu 476100, China
  • Received:2009-12-26 Published:2010-04-28

摘要: 在池栽条件下,研究了灌浆期2个不同基因型冬小麦品种——大穗型品种豫麦66号和多穗型品种豫麦49号籽粒库活性的差异。试验采用KI-I2法测定淀粉含量,参照Douglas等和Tsai等的方法测定SS和AGPP活性,采用Chhaya等的方法测定IAAO活性。结果表明,2个品种籽粒中蔗糖合成酶(SS)活性呈单峰曲线,但豫麦66号的峰值出现偏晚,且高值持续期长;籽粒中吲哚乙酸氧化酶(IAAO)活性变化呈双峰曲线,其峰值分别出现在花后15d和30d;腺苷二磷酸葡萄糖焦磷酸化酶(AGPP)活性呈单峰曲线,均在花后20d达到峰值;豫麦66号淀粉积累高峰出现较晚,但在灌浆中后期淀粉积累量和积累速率均明显高于豫麦49号,显示灌浆后期豫麦66号仍具有较强的库活性。

关键词: 冬小麦, 基因型, 源库关系, 酶活性

Abstract: Two different genotype winter wheat cultivars with large-spike type cultivars Yumai 66 and small-spike type cultivars Yumai 49,were used to study the differences of the sink activity during grain filling stage on the condition of pond culture.Starch content was measured according to the method of KI-I2,SS and AGPP activities were tested according to the method described by Douglas et al and Tsai et al,IAAO activity was measured according to the method of Chhaya et al.The main results showed that the sucrose synthase(SS)activity in the kernel of two cultivars came to be a single-peak curve,but Yumai 66 reached the peak later than that of Yumai 49,and it sustained a long time.Change of indoleacetic acid oxidase(IAAO)activity in the kernel came to be a double-peak curve,its peak appeared in 15 d and 30 d after anthesis,respectively.ADP-glucose pyrophosphorylase(AGPP)activity in the kernel also showed a single-peak curve,the peak appeared in 20 days after anthesis.The peak of starch accumulation of Yumai 66 appeared later than that of Yumai 49,but starch accumulation and starch accumulation rate of Yumai 66 were higher than that of Yumai 49 during middle to latter filling stage,indicating that "Sink" activity of Yumai 66 was stronger than Yumai 49 in the latter filling stage.

Key words: Winterwheat, Geno type, Source-sink relationship, Enzyme activity

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引用本文

王文静, 刘福建. 不同基因型小麦品种灌浆期籽粒库活性的差异[J]. 华北农学报, 2010, 25(2): 160-163. doi: 10.7668/hbnxb.2010.02.032.

WANG Wen-jing, LIU Fu-jian. Studies on the Differences of the Sink Activity in the Kernel During Grain Filling of the Different Genotype Winter Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 160-163. doi: 10.7668/hbnxb.2010.02.032.

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