华北农学报 ›› 2010, Vol. 25 ›› Issue (2): 84-87. doi: 10.7668/hbnxb.2010.02.016

所属专题: 玉米

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玉米生育期QTL定位及上位性互作效应的遗传研究

韩娅楠1, 刘福建2, 王瑞霞3, 丁俊强1, 李志敏1, 李贤唐1, 吴建宇3   

  1. 1. 河南农业大学 农学院, 河南 郑州 450002;
    2. 商丘技师学院, 河南 商丘 476100;
    3. 河南农业大学 生命科学学院, 河南 郑州 450002
  • 收稿日期:2009-11-12 出版日期:2010-04-28
  • 通讯作者: 吴建宇(1964-),男,河南淮阳人,教授,博士生导师,主要从事玉米分子育种等方面的研究。
  • 作者简介:韩娅楠(1984-),女,河南郾城人,在读硕士,主要从事作物遗传育种研究.
  • 基金资助:
    国家高技术研究发展计划项目(“863”计划)(2006AA10Z1D5)

QTL Mapping and Epistasis Analysis of Flowering Related Traits in Maize

HAN Ya-nan1, LIU Fu-jian2, WANG Rui-xia3, DING Jun-qiang1, LI Zhi-min1, LI Xian-tang1, WU Jian-yu3   

  1. 1. Agronomy College of Henan Agricultural University, Zhengzhou 450002, China;
    2. Shangqiu Technician Institution, Shangqiu 476100, China;
    3. Life Sciences College of Henan Agricultural University, Zhengzhou 450002, China
  • Received:2009-11-12 Published:2010-04-28

摘要: 为了探讨玉米生育期的遗传规律,以自交系N6和BT-1为亲本组配了重组自交系(Recombinant inbred line,RIL)群体,利用207个微卫星标记构建分子标记遗传连锁图谱,对生育期相关的抽雄、吐丝和散粉3个性状进行QTL定位,并进行上位性效应分析。结果表明,在第1染色体umc1676-umc1590区域和第2染色体的umc1422-umc1776区域存在共同控制抽雄、吐丝和散粉3个性状的稳定的QTL位点。生育期3个性状QTL的上位性分析,都检测到3对加性×加性上位性互作效应,分别可以解释3.78%~5.43%,1.24%~2.36%和3.27%~4.04%的表型遗传变异。上位性效应是生育期性状的重要遗传基础。

关键词: 玉米, 生育期, 数量性状基因座位, 上位性分析

Abstract: Genetic studies of flowering related traits are important in maize breeding,in this study,a recombinant inbred line(RIL)population derived from the cross between N6 and BT-1 was used for flowering related traits evaluation;a genetic linkage map with 207 SSR markers was constructed for quantitative trait loci(QTL)mapping.The results indicated that stable additive QTL conferring flowering related traits,including days to tasseling,days to silking and days to anthesis were identified on chromosomes 1(bin 1.05-1.06)and 2(bin 2.02)in two environments.For QTL with epistatic effects,3 pairs of epistatic QTL were detected for days to tasseling,days to silking and days to anthesis,respectively,which can account for 3.78%-5.43%,1.24%-2.36% and 3.27%-4.04% phenotypic variation.Epistatic QTL are the important genetic basis for flowering related traits in maize.

Key words: Maize, Flowering related traits, Quantitative trait loci (QTL), Epistasis interaction

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引用本文

韩娅楠, 刘福建, 王瑞霞, 丁俊强, 李志敏, 李贤唐, 吴建宇. 玉米生育期QTL定位及上位性互作效应的遗传研究[J]. 华北农学报, 2010, 25(2): 84-87. doi: 10.7668/hbnxb.2010.02.016.

HAN Ya-nan, LIU Fu-jian, WANG Rui-xia, DING Jun-qiang, LI Zhi-min, LI Xian-tang, WU Jian-yu. QTL Mapping and Epistasis Analysis of Flowering Related Traits in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 84-87. doi: 10.7668/hbnxb.2010.02.016.

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