摘要： 以典型籼稻七山占和典型粳稻秋光构建的F8重组自交系(RIL)为研究对象,选取均匀分布于水稻12条染色体的94对及籼粳特异性28对共122对SSR引物对群体进行标记分析,对与籼粳分类相关的程氏指数各性状进行QTL分析,找出与程氏指数各性状连锁的34对标记,运用这34对标记及28对籼粳特异性标记进行群体籼粳血缘测定,探讨引物类型对分类的影响 并按在每条染色体上均匀选取大约3,5,8对引物共计34,65,90对引物进行群体籼粳血缘测定,探讨引物数量对分类的影响。结果表明:几种方法都将群体划分为以偏粳到粳类型居多的连续分布,籼及偏籼呈极少数。按血缘来源为50%的界限进行籼粳划分对比发现28对籼粳特异标记及34对与程氏指数连锁标记与总122对标记的相符度分别为96%和90%,均匀选取34,65,90对引物与总122对标记的相符度分别为91%,97%和99%,28对籼粳特异标记及65对均匀选取标记与122对标记的分类结果相符度均在95%以上,用较少的引物即可以对群体的籼粳血缘进行分析。
关键词:
水稻,
重组自交系,
分类,
SSR分子标记,
引物类型,
引物数量
Abstract: A recombinant inbred line(R1L)population of F8 generations derived from the cross between qshanzhan(typical indica) and Akihikari (typical japonica) were used as materials. All of 122 SSR markers containing 94 SSR markers which was distributed on 12 chromosome of rice and 28 indica and japonica specific SSR markers were used to markeranalysis. Based on QTL analysis of Cheng's index,34 markers linked to Cheng's index were found. The 34 markers and 28indica and japonica specific SSR markers were conducted to determine the blood relationship of indica and japonica on thepopulation derived from indica and japonica crossing,and to study the effect of markers types on subspecies classificationof indica and japonica. 3,5,8 SSR markers from per chromo summed to 34,65,90 SSR markers were evenly selectedto determine blood relationship of indica and japonica on the population and to study the effect of markers amount on sub-species classification of indica and japonica. The results showed that the distribution of population derived from indica andjaponica crossing were almost continuous distribution keng (japonica) subspecies and japonica. Indica and hsien (indica)were minority. The consistent degree of classification based on the 28 indica and japonica specific SSR markers and 34markers or all of 122 SSR markers were 96%and 90%by the limit of 50%blood relationship of indica and japonica.The consistent degree of classification based on 34,65,90 markers were 91%,97%and 99%.The consistent degree ofclassification based on 28 indica and japonica specific SSR markers and 65 markers or 122 markers were more than 95%.Using few SSR markers can analyse blood relationship of indica and japopica on the population derived from indica and japomca crossing.
Key words:
Rice,
Recombinant inbred line,
Classification,
SSR markers,
Markers type,
Markers amount
中图分类号:
毛艇, 徐海, 郭艳华, 朱春杰, 陈凯, 王嘉宇, 徐正进. 利用SSR分子标记进行水稻籼粳分类体系的初步构建[J]. 华北农学报, 2009, 24(1): 119-124. doi: 10.7668/hbnxb.2009.01.027.
MAO Ting, XU Hai, GUO Yan-hua, ZHU Chun-jie, CHEN Kai, WANG Jia-yu, XU Zheng-jin. Establishment of Subspecies Classification of Indica and Japonica System by SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 119-124. doi: 10.7668/hbnxb.2009.01.027.