摘要： 通过测定2株副溶血性弧菌4~40℃的生长曲线,研究它们的生长规律,运用预测食品微生物学的方法,分别对它们建立初级和二级预测模型。结果表明,副溶血性弧菌的生长曲线在低温阶段,适用线性回归方程拟合;在适温阶段,生长曲线都呈典型的S形,适合用Gompertz模型拟合。二级模型采用平方根方程的扩展式Ratkowsky3式拟合,根据二级预测模型,分别得出副溶血性弧菌的最低生长温度Tmin,最高生长温度Tmax和最适生长温度。模型验证表明,预测值和实测值的残差都在±0.05范围内,偏差因子和准确因子均在可接受范围内,模型是可靠的。最后,讨论了模型的应用前景。

关键词: 预测微生物学, 模型, 副溶血性弧菌, 温度

Abstract: The growth characteristics of two Vibrio parahaemolyticus (V. p for short ) stains were studied by using the method of predicive microbiology. The primary and secondary predictive models of Vibrio Parahaemolyticus were cre?? ated based on their growth conditions at 4- 40. The result showed that the Gompertz model was found to fit with the experimental curves which are typical sigmoidal at fitting temperature and the Monod funct ion were found to f it with the experimental curves at low temperature. The secondary models for the effects of temperaturewere modeled. Data process?? ing of the model has shown the minimum growth temperature, the maximum growth temperature and the optimal growth temperature for two V. p stains. Validation of the models showed that the residual difference of predicted and observed value was within 0.05, and the values of bias factor and accuracy factor were also acceptable statistically. The predictive models are highly reliable. Also, the discussion was given for the application of the predictive model.

Key words: Predictive microbiology, Model, Vibrio parahaemolyticus, Temperature

中图分类号: 

• S941