南美白对虾养殖系统中弧菌为主的致病菌群的分子比较

doi:10.7668/hbnxb.2008.S1.060

华北农学报 ›› 2008, Vol. 23 ›› Issue (S1): 257-262. doi: 10.7668/hbnxb.2008.S1.060

所属专题： 水产；

南美白对虾养殖系统中弧菌为主的致病菌群的分子比较

张洪沂¹, 赵勇¹, 戴习林², 潘迎捷¹

1. 上海水产大学,食品学院,上海,200090;
2. 上海水产大学,生命科学与技术学院,上海,200090

收稿日期:2007-11-18 出版日期:2008-12-31
通讯作者: 潘迎捷(1951- ), 男, 江苏人, 硕士, 博士生导师, 主要从事食用菌遗传育种和农业生物技术研究。
作者简介:张洪沂(1982-),女,河北邯郸人,硕士,主要从事食品生物技术的研究.
基金资助:
上海市科技兴农重点攻关项目(沪农科攻字2005第4-2号;沪农科攻字2006第10-5号);上海市重点学科建设项目(T1102);上海高校选拔培养优秀青年教师科研专项基金(677171401)

Composition of Pathogenic Bacteria in Litopenaeus vannarnei Culture Ecosystem

ZHANG Hong-yi¹, ZHAO Yong¹, DAI Xi-lin², PAN Ying-jie¹

1. College of Food Science,Shanghai Fisheries University,Shanghai 200090,China;
2. College of Aqua-life Science and Technology,Shanghai 200090,China

Received:2007-11-18 Published:2008-12-31

摘要/Abstract

摘要： 弧菌是最常见、最重要的细菌性病原菌,采用16 S rDNA克隆文库法对南美白对虾(Litopenaeus vannarnei)养殖系统中水体和底泥的弧菌为主的致病群落的组成进行分析研究。从水体和底泥16 S rDNA克隆文库中各随机挑选55个克隆子进行测序(约114 bp),对测序结果进行了BLAST比对。结果表明:水体样品有16个OTU,主要Vibrio(弧菌,33.3%)、Chloroflexi(绿屈挠菌,18.5%)和Pantoea(泛菌属菌,7.41%);底泥样品有14个OTU,主要是Vibrio(弧菌,50.0%)和Chloroflexi(绿屈挠菌,13.6%);2个文库比较来看,proteobacterium(变形细菌)仅在水体样品中,Aeromonas(气单胞菌)仅在底泥样品中。用MEGA 4从测序的克隆子中选出30个OTU进行系统发育分析。

关键词: 南美白对虾, 16SrDNA克隆文库, 弧菌属

Abstract: Vibrio is one of the most dominant pathogens. Using 16 S rDNA clone library method, the Vibrio composiion of the Litopenaeus vannarnei Culture Ecosystem was investigated. Compared with the published sequences in Gen-Bank data base, sequencing results of cloned 16 S rDNA amplicons revealed a diverse community in the Litopenaeus vannarnei Culture Ecosystem. The results indicated that Water Sample was clustered into 16 OTUs, the dominant commun-ities are Vibrio, Chlorof lexi and Pantoea, which accounted for 33.3%, 18.5% and 7.41%; sediment samplewas clusteredinto 14 OTUs, the dominant communities are Vibrio and Pantoea, which accounted for 50.0% and 13.6%, respectively.The results had a little difference from the two samples: there were not Aeromonas in the Water Sample andthere were not proteobacterium in the sediment sample.

Key words: Litopenaeus vannamei , 16 S rDNA clone library, Vibrio genus

中图分类号:

S945

张洪沂, 赵勇, 戴习林, 潘迎捷. 南美白对虾养殖系统中弧菌为主的致病菌群的分子比较[J]. 华北农学报, 2008, 23(S1): 257-262. doi: 10.7668/hbnxb.2008.S1.060.

ZHANG Hong-yi, ZHAO Yong, DAI Xi-lin, PAN Ying-jie. Composition of Pathogenic Bacteria in Litopenaeus vannarnei Culture Ecosystem[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S1): 257-262. doi: 10.7668/hbnxb.2008.S1.060.

http://www.hbnxb.net/CN/Y2008/V23/IS1/257

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南美白对虾养殖系统中弧菌为主的致病菌群的分子比较

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