摘要： 旨在为葡萄生产栽培提供理论依据,通过氮肥不同施用量处理,研究探讨了氮肥对盆栽葡萄叶片生长及根、叶细胞结构的影响。结果表明,随着氮肥施用量的增加,葡萄叶片的长和宽及其叶面积都呈现增大趋势,叶绿素含量也显著增加,其中,叶绿素a增加显著,而叶绿素b增加不显著,同时,叶绿素荧光量子吸收率显著增加,且与叶绿素a/b值显现出一致的趋势,说明叶绿素俘获光量子与叶绿素a有很大关系。通过切片观察,随着氮肥施用量的增加,DAPI染色后,对叶片细胞核影响不显著,但在根尖部,细胞核数量和染色程度显著增加;经I2-KI染色显示,随着氮肥施用量的增加,葡萄叶片、根尖中淀粉粒积累也呈现增加的趋势。

关键词: 葡萄, 氮肥, 叶片, 根

Abstract: Nitrogen fertilizer effects on the grape leaves′ growth and the anatomical changes both of the leaves and roots through different levels of nitrogen fertilizer applications.The results express that the leaves′ length,width and leaf area are increased with the amount of nitrogen fertilizer applications′ increasing,which is also same to the content of chlorophyll,Especially,chlorophyll a increased sharply,while the chlorophyll b didn′t increase in evidence.Then the chlorophyll fluorescence quantum absorptivity is increased strongly,which is same with the chlorophyll a/b′s trend of change.That indicates quantum absorptivity is in relation with the chlorophyll a.Samples are observed by the cross sections,after the staining by DAPI,as the amount of nitrogen fertilizer applications increasing,nucleolus amount of the leaves′ is not changed in evidence,while the nucleolus amount in the root apiex meristem is increased sharply.After the staining by the I2-KI,also the starch content of both the leaves and roots is increased in evidence as the amount of nitrogen fertilizer applications increasing.

Key words: Grape, Nitrogen fertilizer, Leaf, Root

中图分类号: 

• S663.1