华北农学报 ›› 2007, Vol. 22 ›› Issue (4): 5-8. doi: 10.7668/hbnxb.2007.04.002

所属专题: 水稻 生物技术

• 论文 • 上一篇    下一篇

水稻粳型亲籼系S-c座位基因型分析

丁效华, 张泽民, 曾瑞珍, 张桂权   

  1. 华南农业大学, 广东省植物分子育种重点实验室, 广东 广州 510642
  • 收稿日期:2007-06-15 出版日期:2007-08-28
  • 通讯作者: 张桂权(1957-),男,广东高要人,博士,教授,主要从事植物分子育种研究。
  • 作者简介:丁效华(1962-),男,江西南城人,博士,研究员,主要从事植物分子育种研究。
  • 基金资助:
    国家自然科学基金(30471068);广东省科学技术项目资助(2004B20101003)

Genotypic Identification of the S-c Locus in the Indica-compatible Japonica Lines of Rice (Oryza sativa L.)

DING Xiao-hua, ZHANG Ze-min, ZENG Rui-zhen, ZHANG Gui-quan   

  1. Guangdong Provinicial Key Lab of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China
  • Received:2007-06-15 Published:2007-08-28

摘要: S-c是控制水稻籼粳亚种间杂种F1花粉不育性的基因座位之一。在该座位,台中65的基因型为Sj/Sj,广陆矮号的基因型为Si/Si。以台中65和广陆矮号为遗传测验种,分别与个粳型亲籼系配组,根据部分F2群体中植株花粉育性表型及与S-c紧密连锁分子标记基因型的偏态分离程度,测定了这个粳型亲籼系在S-c座位的基因型,结果表明,G216-3的基因型为Si-2/Si-2;G2605和G300-的基因型均为Si-1/Si-1;G217-2-1的基因型为Sn/Sn。本文还对F1花粉不育性基因遗传分化的测定方法进行了讨论。

关键词: 水稻, 粳型亲籼系, 花粉不育性, 复等位基因, 微卫星标记

Abstract: S-c is one of the F1 pollen sterility loci between indica and japonica cultivars in rice. The genotype of Taichong 65 (T65) and Guangluai 4 (GLA 4) at the locus is Sj/Sj and Si/Si, respectively. Four indica-compatible japonica lines (ICJLs), G24163, G2417-2-1, G2605 and G3004-4 were crossed with testers, T65 and GLA 4, and their S-c locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-c locus and plant pollen fertility in several F2 populations showed that G2416-3 possessed Si-2/Si-2 genotype; G2605 and G3004-4 possessed Si-1/Si-1 genotype; while G2417-2-1 possessed Sn/Sn genotype. The test methods on genetic differentiation of the F1 pollen sterility loci were also discussed in this paper.

Key words: Rice (Oryza sativa L.), Indica-compatible japonica lines, Pollen sterility, Multi-alleles, Microsatellite marker

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引用本文

丁效华, 张泽民, 曾瑞珍, 张桂权. 水稻粳型亲籼系S-c座位基因型分析[J]. 华北农学报, 2007, 22(4): 5-8. doi: 10.7668/hbnxb.2007.04.002.

DING Xiao-hua, ZHANG Ze-min, ZENG Rui-zhen, ZHANG Gui-quan. Genotypic Identification of the S-c Locus in the Indica-compatible Japonica Lines of Rice (Oryza sativa L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 5-8. doi: 10.7668/hbnxb.2007.04.002.

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