华北农学报 ›› 2007, Vol. 22 ›› Issue (4): 1-4. doi: 10.7668/hbnxb.2007.04.001

所属专题: 玉米 生物技术

• 论文 •    下一篇

ENR基因在高油和普通玉米自交系中的克隆和表达分析

柳展基, 唐桂英, 单雷, 毕玉平   

  1. 山东省农科院高新技术研究中心, 山东省作物与畜禽品质改良生物技术重点实验室, 山东 济南 250100
  • 收稿日期:2007-04-11 出版日期:2007-08-28
  • 作者简介:柳展基(1972-),男,山东龙口人,博士,助理研究员,主要从事植物分子生物学研究。
  • 基金资助:
    国家自然科学基金项目(30571165)

The Cloning and Expression Analysis of ENR Gene of High-Oil and Normal Inbred Lines in Maize

LIU Zhan-ji, TANG Gui-ying, SHAN Lei, BI Yu-ping   

  1. Hi-Tech Research Centre, Shandong Academy of Agriculture Sciences, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Jinan 250100, China
  • Received:2007-04-11 Published:2007-08-28

摘要: 利用高油玉米自交系By80和普通玉米自交系B73为材料,克隆Enoyl-ACP reductase(ENR)基因,同时比较ENR基因的序列和表达差异。结果表明,ENR基因编码蛋白在高油和普通玉米中氨基酸序列完全一致;在授粉后15,25和35 d的胚中,ENR基因呈下调表达,在同一发育时期,ENR基因在高油玉米By80中较高表达。

关键词: 高油玉米, ENR基因, 表达分析

Abstract: High-oil inbred line By804 and normal inbred line B73 were utilized to clone the gene of enoyl-ACP reductase (ENR) and then to discriminate the difference of sequence and expression at RNA level of ENR in By804 and B73. The results indicated there was no difference in amino acid sequence of ENR in By804 and B73; the expression at RNA level was down-regulated at 15, 25 and 35 d after pollination (DAP) in both By804 and B73 and the expression of ENR in By804 was higher than that in B73 at the same embryo development stage.

Key words: High-oil maize, ENE gene, Expression analysis

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引用本文

柳展基, 唐桂英, 单雷, 毕玉平. ENR基因在高油和普通玉米自交系中的克隆和表达分析[J]. 华北农学报, 2007, 22(4): 1-4. doi: 10.7668/hbnxb.2007.04.001.

LIU Zhan-ji, TANG Gui-ying, SHAN Lei, BI Yu-ping. The Cloning and Expression Analysis of ENR Gene of High-Oil and Normal Inbred Lines in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 1-4. doi: 10.7668/hbnxb.2007.04.001.

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