摘要： 以水稻核基因型为协青早的4种同核异质雄性不育细胞质(D协青早A,G协青早A,T协青早A,W协青早A)及其保持系正常细胞质(协青早B)的一叶一心期幼苗为试材,通过对野败性雄性不育细胞质具有专化侵染性的稻瘟病菌90-2菌株产生的粗毒素浸根处理4 h后,用等电聚焦电泳法测定叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、醋酶同工酶谱的变化,比较分析了各同核异质不同细胞质对处理反应的病理生理差异。结果显示,同核情况下不同细胞质试材的SOD、POD和酯酶同工酶谱存在不同程度的差异,毒素处理后其同工酶谱的反应也存在不同程度的区别,细胞质的不同除引起SOD、POD等的酶活性存在差异外,对SOD、POD和酯酶同工酶谱也有一定的影响,表明POD同工酶谱的变化与抗性有一定关系。

关键词: 水稻, 细胞质雄性不育, 毒素, 稻瘟病, 酯酶同工酶, 过氧化物酶同工酶, 超氧化物, 歧化酶同工酶

Abstract: The seedlings of four kinds of rice Xieqingzao CMS and their normal cytoplasm with homocaryon,used as experimental materials, were treated soakedly by crude toxins from P. oryzae 90-2 strain which was special pathogenic to wild abortive rice CMS. After four hours of treat-ment,IEF was utilited to detect their changed states of SOD,POD and esterase isozymes in their leaves in order to analysis the differences of pathologic reaction among them. The results showed that differences of these isozyme types in homocaryon CMS existed to some extent. The experi-ments revealed that their reactions of activities and isozyme types to the toxins were different. The experiment can obtain the conclusion that the change of POD isozyme types is related to resis-tance of CMS to the toxins in rice.

Key words: Pyricularia oryzae, toxin, Rice, Male-sterile cytoplasm, Peroxidase, Superoxidase dis-mutase, Esterase, Isozyme

中图分类号: 

• S435.111.49