摘要： 对王林、乔纳金、长富2、秋富1、M7、M26六个苹果品种及砧木的脱毒与未脱毒苗木进行测定，结果显示，脱毒后成叶中过氧化物酶活性显着降低。王林、长富2的脱毒苗成叶内硝酸还原酶活性比对照极显着增强。王林、乔纳金、长富2三品种脱毒苗成叶中蛋白质及氨基酸含量显着降低，幼叶及嫩梢皮层中蛋白质含量显着增高。脱毒后，三个品种成叶组织提取液褐变度明显降低。

关键词: 苹果, 脱毒, 过氧化物酶, 硝酸还原酶, 蛋白质, 氨基酸, 褐变度

Abstract: In six apple strains or stocks, including Orin, Jonagold, Nagafu 2, Akifu 1, M7 and M26, the peroxidase activity in the mature leaves of the virus-free seedlings were obviously decreased, compared with oh (viruliferous ones). The nitrate reductase activity in the mature leaves of virus-free Orin, Jonagold and Nagafu 2 was also remarkably higher than their oh. The contents of protein and amino acid in the mature leaves of the three strains were much lower, but in the browses and the shoot cortex were higher, and nitrite concentration in them was decreased. After virus-free treatment the browning degree of the tissues extraction of the three strains was notably decreased.

Key words: Apple, Virus-free treatment, POD, Nitrate reductase, Protein, Amino acids, Browning degree

中图分类号: 

• S661.101