黄萎病菌胁迫下陆地棉冀2658根部蛋白质组研究

doi:10.7668/hbnxb.20190703

摘要/Abstract

摘要： 为了挖掘陆地棉黄萎病菌侵染应答相关基因，揭示陆地棉应对黄萎病菌侵染的应急响应机制。以抗黄萎病品种冀2658为材料，用大丽轮枝菌Vd991侵染1 h的棉花根部为样品，以水处理做对照，利用同位素标记相对和绝对定量（Isobaric tags for relative and absolute quantification，iTRAQ）技术进行蛋白质组研究。结果共筛选到46个差异表达蛋白质，37个上调表达，9个下调表达。对差异蛋白进行基因本体（Gene ontology，GO）注释分析，发现差异蛋白的功能分为24类，分子功能占7类，生物过程占8类，细胞组分占9类。KEGG Pathway分析发现，差异表达蛋白参与32个代谢通路，其中苯丙氨酸代谢P值（0.025 308 8）最小，达到显著水平。通过分析差异表达蛋白的功能，发现在黄萎病菌胁迫早期（1 h），能量代谢相关蛋白、组织结构代谢相关蛋白、蛋白质合成相关蛋白、抗氧化物酶类、核苷二磷酸激酶等参与到棉花抵抗黄萎病的过程。并且，细胞色素C（Cyt-c）的释放说明棉花根部启动程序性细胞死亡（Programmed cell death，PCD）来抑制黄萎病菌的传播。

关键词: 陆地棉, 根系, 黄萎病, 蛋白质组, 抗病机制

Abstract: In order to discover the genes related to the infection response of Verticillium dahliae in upland cotton and to reveal the emergency response mechanism of upland cotton to Verticillium dahliae infection,the proteome of cotton roots under Verticillium wilt stress was studied by iTRAQ(Isobaric tags for relative and absolute quantification,iTRAQ),using Ji 2658 as the material,and cotton roots were infected with Verticillium dahliae Vd991 for 1 h as the sample,and water treatment as the control.Forty-six differentially expressed proteins(DEPs)were screened,of which 37 were up-regulated and 9 were down-regulated.Gene ontology annotation analysis showed that the functions of DEPs were divided into 24 categories,with molecular functions accounting for 7 categories,biological processes accounting for 8 categories and cell components accounting for 9 categories.Pathway analysis revealed that DEPs were involved in 32 metabolic pathways.The metabolic P value of phenylalanine metabolism(0.025 308 8)was the smallest.By analyzing the functions of DEPs,it was found that energy metabolism-related proteins,tissue metabolism-related proteins,protein synthesis-related proteins,antioxidant enzymes,nucleoside diphosphate kinase(NDPK)were involved in the process of cotton resistance to Verticillium wilt in the early stage of Verticillium wilt stress(1 h).Moreover,the release of cytochrome c(Cyt-c)indicated that cotton root initiated programmed cell death(PCD)to inhibit the transmission of Verticillium wilt.

Key words: Upland cotton, Root, Verticillium wilt, Proteome, Disease resistance mechanism

中图分类号:

S432.4

李丹, 赵存鹏, 刘素恩, 王凯辉, 王兆晓, 刘旭, 郭宝生, 耿军义. 黄萎病菌胁迫下陆地棉冀2658根部蛋白质组研究[J]. 华北农学报, 2020, 35(S1): 322-331. doi: 10.7668/hbnxb.20190703.

LI Dan, ZHAO Cunpeng, LIU Suen, WANG Kaihui, WANG Zhaoxiao, LIU Xu, GUO Baosheng, GENG Junyi. Proteomic Studies on Roots of Upland Cotton Ji 2658 under Verticillium dahliae Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 322-331. doi: 10.7668/hbnxb.20190703.

http://www.hbnxb.net/CN/Y2020/V35/IS1/322

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