摘要： 取生殖期大蒜花梗,在N₆+2,4-D 2mg/L+KT 1mg/L培养基上暗培养30天后,得到微黄色愈伤组织。将愈伤组织转到N₆+2,4-D 0.5mg/L+KT 0.5mg/L培养基上继代二次,然后于N₆+BA 5mg/L+KT 1mg/L+IAA 0.01mg/L,MS（1/2大量元素）+BA 5mg/L+KT 1mg/L+IAA 0.01mg/L和MS+BA 5mg/L+KT 1mg+IAA 0.01mg/L培养基上光照培养。3周后愈伤组织上出现绿色丛生芽,将小芽转到MS（1/2大量元素）+NAA 0.01mg/L培养基上,一周后发生白色根并形成完整植株。2,4-D和KT对大蒜花梗愈伤组织发生是必要的,高浓度的细胞分裂素和低浓度的生长素有利于芽分化,高浓度NH₄⁺有利于根分化,低浓度NH₄⁺有利于芽分化。愈伤组织低温处理对芽的分化是必要的。另外,对花梗愈伤组织发生进行了细胞学观察。

关键词: 大蒜, 花梗, 再生植株, 植物激素

Abstract: Calli appeared from pedicels of garlic cultured on N8+2,4-D2mg/L+KTlmg/L for 30 days, These calli survived two subcultures on N8+2,4-D 0,5mg/L+KT 0.5mg/L,Organogenesis occurred on NB+BA 5mg/L+KT lmg/L+IAA O,Olmg/L MS(令Macroelements)+BA 5mg/L+KT 1mg/L+IAA O.Olmg/L and MS+BA 5mg/L+KT lmg/L+IAA 0.01mg/L, Roots appeared one week after the shoot was transferred onto the MS(专Macroelements)+NAA O.Olmg/L medium2,4-D and KT were essential to the induction of calli from garlic pedicels, Higher concentrations of plant growth regulators，i.e,cytokinin/auxin, lower concentration of NH李and cold treatment to calli were all essential to shoot formation, Cellular observation on the process of callus induction from garlic pedicel is also, disscussed.

Key words: Garlic, Pedicel, Plant regeneration, Plant growth regulators