华北农学报 ›› 1992, Vol. 7 ›› Issue (3): 59-65. doi: 10.3321/j.issn:1000-7091.1992.03.012

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固体培养基上浸润培养提高花粉植株诱导率的研究

王培, 陈玉蓉   

  1. 河北省农科院粮油作物研究所, 石家庄 050031
  • 收稿日期:1991-01-26 出版日期:1992-09-28

A Study Plants Increasing Induction Frequency of Pollen Culture with a Solidified Thin Liquid Layer on Medium

Wang Pei, Chen Yurong   

  1. Cereal and Oil Crops Institute, Hebei Academy of Agricultural and ForestrySciences, Shijiazhuang 050031
  • Received:1991-01-26 Published:1992-09-28

摘要: 在30×170mm的试管内,倒入25~30ml加琼脂固化的C17培养基,接种冬小麦花药后注入2ml左右不加琼脂而浓度相同的C17培养液浸润培养,冬小麦花药愈伤组织诱导率可从固体培养基的13.94%提高到29.95%,愈伤组织的绿苗分化率还比固体培养基提高10.2%,分化绿苗丛数占接种花药的10.94%,是固体培养的2.37倍。

关键词: 冬小麦, 花药培养, 浸润, 固体培养

Abstract: After inoculation of winter wheat anther into glass tubes containing 25 to 30 ml agar-solidified C,7medium, about 3 mm lipuid medium was added. This raised the callus induction frequency of winter wheat anthes from 13,94%to 29,95%and the green plant regeneration frequency from calli by 1U.2)%in comparison with culture on solidified media, By this method the number of regenerate」green plants accounted for 10.94%of the inoculated anthers which、-as 2.73 times as much as that by culture‘n solidified medium.

Key words: v inter wheat, Anther calcure, Solidified

引用本文

王培, 陈玉蓉. 固体培养基上浸润培养提高花粉植株诱导率的研究[J]. 华北农学报, 1992, 7(3): 59-65. doi: 10.3321/j.issn:1000-7091.1992.03.012.

Wang Pei, Chen Yurong. A Study Plants Increasing Induction Frequency of Pollen Culture with a Solidified Thin Liquid Layer on Medium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1992, 7(3): 59-65. doi: 10.3321/j.issn:1000-7091.1992.03.012.

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