摘要： 利用组织培养对内蒙古特色资源的河套苹果梨进行遗传保护、品质改良以及生物技术操作具有重要的理论意义和实践价值.试验利用NAA、2，4-D和6-BA对苹果梨叶片进行组织脱分化研究.结果表明，0～0.2 mg/L 的NAA与0.1 mg/L 的6-BA组合可有效促进叶片组织脱分化，形成愈伤组织或直接诱导叶芽的萌发.NAA与2，4-D组合未表现出有明显促进河套苹果梨叶片组织脱分化的作用.在叶片组织脱分化过程中，外植体的染菌率与激素水平存在一定的线性关系.

Abstract: In order to accomplish the research project of Hetao apple-pear's tissue culture,this study conducted a primary study of tissue dedifferentiation of Hetao apple-pear under the effects of the three plant hormones NAA,2,4-D and 6-BA.The results indicated that the effects of combination of NAA(0-0.2 mg/L) and 6-BA(0.1 mg/L) can effectively dedifferentiate the vegetative tissue(buds) of Hetao apple-pear,and stimulated callus formation or directed growth of leave buds.The combinations of NAA and 2,4-D had potential effects on tissue dedifferentiation of Hetao apple-pear.Meanwhile,it was found that the intensity of microorganism contamination of explants during tissue culturing were correlated to the concentration of plant hormone.

Key words: Hetao apple_pear, Plant hormone, Tissue dedifferentiat ion, Combined effects

中图分类号: 

• S661.22