华北农学报 ›› 2006, Vol. 21 ›› Issue (2): 66-69. doi: 10.3321/j.issn:1000-7091.2006.02.016

• 论文 • 上一篇    下一篇

利用高效液相色谱筛选产植物激素细菌

连翠飞1,2, 蒋继志1, 李社增2, 鹿秀云2, 晁春燕1, 马平2   

  1. 1. 河北大学 河北保定 071002;
    2. 河北省农林科学院植物保护研究所, 河北保定 071000
  • 收稿日期:2006-01-20 出版日期:2006-04-28
  • 通讯作者: 马平
  • 作者简介:连翠飞(1981- ),女,河北石家庄人,在读硕士,主要从事植物病害生物防治研究工作。
  • 基金资助:
    河北省农林科学院重点项目资助(A03-1-03-09)

Screening Bacterium Producing Auxin Through High Performance Liquid Chromatography (HPLC)

LIAN Cui-fei1,2, JIANG Ji-zhi1, LI She-zeng2, LU Xiu-yun2, CHAO Chun-yan1, MA Ping2   

  1. 1. Hebei University, Baoding 071002, China;
    2. Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, China
  • Received:2006-01-20 Published:2006-04-28

摘要: 利用C18色谱柱,以甲醇、水、冰乙酸(45:54.8:0.2)为流动相于255nm检测细菌培养液提取物,根据HPLC检测结果快速筛选得到既产赤霉素(GA3)又产生长素(IAA)的细菌1株CX-5-2,并利用HPLC进一步研究并明确了该菌株不产植物激素的适宜条件。同时明确了该菌株对小麦生长具有促进的潜力。

关键词: 高效液相色谱, 细菌, 植物激素, 赤霉素, 生长素

Abstract: The anxin including GA3 and IAA in the culture broth of 300 bacterial isolates were measured through HPLC in this study,and an isolate CX-5-2 were screend and determined to prorduce GA3 and IAA.Simultaneously,the optimum conditions of strain CX-5-2 to produce auxin were studied through HPLC.The results from the experiment on effects of strain CX-5-2 culture broth on the groth of wheat seedlings determined that this bacterial strain has the potential ability of plant growth-promotion.

Key words: HPL, Bacterium, Auxin, GA3, IAA

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引用本文

连翠飞, 蒋继志, 李社增, 鹿秀云, 晁春燕, 马平. 利用高效液相色谱筛选产植物激素细菌[J]. 华北农学报, 2006, 21(2): 66-69. doi: 10.3321/j.issn:1000-7091.2006.02.016.

LIAN Cui-fei, JIANG Ji-zhi, LI She-zeng, LU Xiu-yun, CHAO Chun-yan, MA Ping. Screening Bacterium Producing Auxin Through High Performance Liquid Chromatography (HPLC)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 66-69. doi: 10.3321/j.issn:1000-7091.2006.02.016.

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