大白菜CMS7311雄性不育的发生与花蕾内源激素含量变化的关系研究

doi:10.7668/hbnxb.2014.06.030

华北农学报 ›› 2014, Vol. 29 ›› Issue (6): 177-182. doi: 10.7668/hbnxb.2014.06.030

所属专题： 白菜；

大白菜CMS7311雄性不育的发生与花蕾内源激素含量变化的关系研究

许小勇^1,2, 张静^1,2, 孙希禄², 李梅兰¹, 雷鸣³, 张鲁刚²

1. 山西农业大学园艺学院, 山西太谷 030801;
2. 西北农林科技大学园艺学院, 陕西杨陵 712100;
3. 西北农林科技大学生命科学学院, 陕西杨陵 712100

收稿日期:2014-09-14 出版日期:2014-12-28
通讯作者: 张鲁刚(1963-),男,陕西岐山人,教授,博士,博士生导师,主要从事大白菜种质资源创新与新品种选育研究。
作者简介:许小勇(1979-),男,江苏海安人,讲师,博士,主要从事蔬菜种质资源创新与遗传育种研究。
基金资助:
国家自然科学基金项目(30871717);国家重大科学仪器开发专项(2012YQ030261);山西省科技农业攻关项目(20140311009-3);山西农业大学科研启动经费(2013YJ46)

The Relationship between Male Sterile Occurrence and Dynamics of Endogenous Hormone Contents during Flower Bud Development in CMS7311 of Heading Chinese Cabbage

XU Xiao-yong^1,2, ZHANG Jing^1,2, SUN Xi-lu², LI Mei-lan¹, LEI Ming³, ZHANG Lu-gang²

1. College of Horticulture, Shanxi Agricultural University, Taigu 030801, China;
2. College of Horticulture, Northwest A & F University, Yangling 712100, China;
3. College of Life Sciences, Northwest A & F University, Yangling 712100, China

Received:2014-09-14 Published:2014-12-28

摘要/Abstract

摘要： 为揭示大白菜CMS7311雄性不育发生与其内源激素含量变化的关系,利用间接酶联免疫(ELISA)测定法比较了大白菜雄性不育株花蕾和可育株花蕾发育过程中内源激素的含量变化差异。结果表明:在花蕾发生过程中,不育株和可育株内源激素IAA、ABA、GA₃、ZR以及JA含量变化均呈现差异。以花蕾纵径2.0~2.9 mm时期为转折点,前期不育株花蕾IAA含量小于可育株花蕾,而后期相反;同样的转折点,不育株花蕾ABA含量的下降趋势呈开张的“V”字形,前期下降快,后期下降放缓,这与可育株中变化趋势不同。不育和可育株花蕾中GA₃含量变化都是先下降后上升趋势,所不同的是不育株花蕾中GA₃含量变化幅度较可育株小且始终低于可育株。不育株中JA含量呈现先下降后上升再下降的剧烈变化趋势,且始终高于可育株花蕾中的JA含量。在不育株不育发生的初始阶段,ABA和JA分别上升了12.47%和16.18%,而IAA、ZR和GA₃分别下降了14.00%,23.04%和45.68%;IAA/ABA呈现先下降后上升趋势,但在花蕾发育早期不育株中的比值小于可育株,而后期正好相反;其余IAA/GA₃、IAA/ZR和ABA/GA₃ 等3类激素比值均表现为下降趋势,且不育株中IAA/GA₃和ABA/GA₃比值都明显大于可育株。由于大白菜CMS雄性不育主要发生在花蕾发育的早期,推断GA₃的缺乏,导致各激素间的平衡关系失调,可能与CMS7311雄性不育的发生有关。

关键词: 大白菜, 雄性不育, 花蕾, 植物激素

Abstract: To reveal the relationship between the occurrence of male sterility of CMS7311 and endogenous hormone levels.In this research,enzyme-linked immunosorbent assay(ELISA)method was used to compares the dynamics of endogenous hormone contents of the flower buds between male sterile and fertile plants in heading Chinese cabbage.The results showed that the content of endogenous hormones IAA,ABA,GA₃,ZR and JA were significant different dynamics both in sterile plants and fertile plants during the flower bud development.Using the flower bud with the size of 2.0-2.9 mm as the turning point,the IAA contents of prior stages in the sterile bud were less than that of the fertile bud,and were contrary at the later stages.With the same turning point,the declining dynamics of ABA contents showed an opening “V” glyph which declining fast at the earlier stages and slow in the late stages,this was just different from the trends in fertile plants.The dynamics of GA₃ contents both in the sterile and fertile flower buds were all declining and then rising,the difference was that the dynamic range of GA₃ content in sterile buds showed smaller and the GA₃ content were always lower than that in fertile plants.The dynamics of JA contents in the sterile flower buds showed the violent change of decline,rise and decline,and was always higher than that in fertile buds.In the initial stage of male sterile occurring,ABA and JA were increased by 12.47% and 16.18%,while the IAA,ZR and GA₃ decreased by 14.00%,23.04% and 45.68% than that in male fertile buds.IAA/ABA ratio showed a fluctuating dynamics which downward after the first rise in both flower buds.The difference was that IAA/ABA ratio in the bud early stage of male sterile plants was less than that in fertile plants,The rest three ratio,including IAA/GA₃,IAA/ZR and ABA/GA₃,showed the declining dynamics,and the ratios of IAA/GA₃ and ABA/GA₃ in male sterile plants were significantly higher than that in fertile plants.For the male sterility mainly occurred at the early stages of flower buds in CMS of heading Chinese cabbage,we concluded that the lack of GA₃ which resulted into the unbablance of each endogenous hormone,may be associated with the occurrence of male sterility in CMS7311.

Key words: Heading Chinese cabbage, Male sterility, Flower Buds, Phytohormone

中图分类号:

S634.1

许小勇, 张静, 孙希禄, 李梅兰, 雷鸣, 张鲁刚. 大白菜CMS7311雄性不育的发生与花蕾内源激素含量变化的关系研究[J]. 华北农学报, 2014, 29(6): 177-182. doi: 10.7668/hbnxb.2014.06.030.

XU Xiao-yong, ZHANG Jing, SUN Xi-lu, LI Mei-lan, LEI Ming, ZHANG Lu-gang. The Relationship between Male Sterile Occurrence and Dynamics of Endogenous Hormone Contents during Flower Bud Development in CMS7311 of Heading Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 177-182. doi: 10.7668/hbnxb.2014.06.030.

http://www.hbnxb.net/CN/Y2014/V29/I6/177

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