摘要： 为了在辣椒三系杂交育种研究中缩小群体筛选范围，减小工作量，提高不育系和保持系选择效率，根据细胞质育性标记SCAR130的序列多态性位点设计KASP标记引物，使其转化为KASP130分子标记，并以辣椒三系材料的雄性不育系、保持系、恢复系和F₁杂交种为试验对象，利用荧光定量PCR仪LC480和LGC公司的SNPline这两种检测平台将KASP130标记应用于辣椒细胞质类型检测，并对该标记稳定性和可靠性进行了检验。结果表明，KASP130标记同SCAR130标记一样可以把待试辣椒材料准确地分为可育细胞质（N）和不育细胞质（S），并在分子标记辅助选择育种中成功应用到辣椒细胞质育性的早期鉴定以及辣椒保持系和雄性不育系的回交育种研究。综上，细胞质育性标记SCAR130已经被成功转化为KASP130分子标记，该标记可以明确鉴定辣椒的细胞质类型，这为其在辣椒三系杂交育种上的应用奠定了研究基础。

关键词: 辣椒, 胞质雄性不育, 三系配套, 竞争性等位基因特异性PCR

Abstract: In order to narrow population range to be screened, reduce the workload and improve the selection efficiency of sterile and maintainer lines in the three-line cross breeding of pepper, the sequence polymorphism sites of cytoplasmic fertility marker SCAR130 was used to design KASP primer to transform SCAR130 into KASP130 molecular marker. The three lines material (male sterile line, maintainer line, restorer line) and F₁ hybrids of pepper CMS were selected as the experimental subjects, and the KASP130 molecular marker were applied into detection of pepper cytoplasm type and its stability and reliability were tested through two detection platforms of real time PCR machine LC480 and the SNPline of LGC Company, respectively. The results showed that KASP130 marker, same as SCAR130, could accurately divide the tested pepper materials into fertile cytoplasm(N) and sterile cytoplasm(S). Furthermore, in the marker-assisted selection breeding, KASP130 was also successfully applied to the early identification of cytoplasmic fertility of pepper as well as the backcross breeding of pepper maintainer lines and male sterile lines. In conclusion, cytoplasmic fertility marker SCAR130 has been successfully transformed into KASP130 molecular marker. It can identify the cytoplasm type of pepper, which lays a foundation for application of KASP130 in three-line cross breeding programe of peppers.

Key words: Pepper, Cytoplasmic male sterily(CMS), Three-line seed production system, KASP

中图分类号: 

• S641.03