华北农学报 ›› 2020, Vol. 35 ›› Issue (4): 71-78. doi: 10.7668/hbnxb.20190723

所属专题: 甜椒辣椒 生物技术

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

辣椒抗坏血酸生物合成酶GME基因家族 的鉴定和表达分析

罗建, 张国斌, 车旭升, 秦启杰   

  1. 甘肃农业大学 园艺学院, 甘肃 兰州 730070
  • 收稿日期:2020-05-20 出版日期:2020-08-28
  • 通讯作者: 张国斌(1977-),男,甘肃武威人,副教授,博士,主要从事蔬菜生长与生理调控研究。
  • 作者简介:罗建(1994-),男,甘肃张掖人,在读硕士,主要从事设施蔬菜栽培研究。
  • 基金资助:
    甘肃省科技重大专项计划(17ZD2NA015-02);甘肃省自然科学基金(17JR5RA156)

Bioinformation Analysis and Expression Analysis of GME Genes Family in Ascorbic Acid Biosynthesis of Pepper

LUO Jian, ZHANG Guobin, CHE Xusheng, QIN Qijie   

  1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2020-05-20 Published:2020-08-28

摘要: GDP-甘露糖-3',5'-异构酶基因(GME)是抗坏血酸途径中的关键限速酶,该基因可以调节AsA合成。为了解辣椒中GME基因家族功能及在非生物胁迫下的表达情况,通过生物信息学方法对辣椒GME基因家族进行鉴定,并对基因结构、结构域、系统进化关系、基因表达模式和非生物胁迫表达情况进行分析。结果表明,在辣椒中有2个GME家族成员,CaGME1CaGME2,分别分布在3号和8号染色体上,都有6个外显子;CaGME1和CaGME2蛋白均具有NADB Rossmann家族中高度保守的NADPH结合位点、底物特异性结合位点,均不具有信号肽,为亲水性蛋白,定位在细胞质粒的可能性最高,均不具有跨膜结构,二级结构主要以不规则卷曲和α-螺旋为主;系统进化树分析表明,该基因编码的氨基酸序列与番茄和马铃薯的亲缘关系最近;qRT-PCR分析结果表明,在低温、茉莉酸甲酯和盐胁迫下CaGME1CaGME2均被诱导上调,在赤霉素下均被诱导下调。综上可知,CaGME1CaGME2基因在胁迫下不同的表达情况表明其在非生物胁迫中发挥作用,可为辣椒逆境胁迫机制研究提供参考。

关键词: 辣椒, 抗坏血酸, GME基因家族, 表达分析

Abstract: The GDP-mannose-3',5'-isomerase gene (GME) is a key rate-limiting enzyme in the ascorbate pathway that regulates AsA synthesis. In order to understand the function of GME gene family in pepper and its expression under abiotic stress,it provides a theoretical basis for the research of pepper resistance. This study identified the GME gene family of pepper by bioinformatics method,and analyzed the gene structure,domain,phylogenetic relationship,gene expression pattern and abiotic stress expression. The results showed that there were 2 GME family members in the pepper, CaGME1 and CaGME2,which were distributed on chromosomes 3 and 8,respectively,and both the two genes had 6 exons. CaGME1 and CaGME2 protein had highly conserved NADPH binding sites and substrate-specific binding sites in the NADB Rossmann family. The two GME proteins had zero signal peptide and transmembrane structure,belong to hydrophilic protein,it was most likely to be localized in a cell plasmid,and the secondary structure of the protein had Alpha helix,Beta turn and Random coil. Phylogenetic tree analysis showed that the amino acid sequence encoded by the two genes was closely related to tomato and potato. qRT-PCR analysis showed that, CaGME1 and CaGME2 were up-regulated under low temperature,methyl jasmonate and salt stress,induced by gibberellin. In summary,the different expressions of CaGME1 and CaGME2 genes under stress indicate that they play a role in abiotic stress,which can provide a reference for the research of pepper stress mechanism.

Key words: Pepper, L-ascorbic acid, GME gene family, Expression analysis

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引用本文

罗建, 张国斌, 车旭升, 秦启杰. 辣椒抗坏血酸生物合成酶GME基因家族 的鉴定和表达分析[J]. 华北农学报, 2020, 35(4): 71-78. doi: 10.7668/hbnxb.20190723.

LUO Jian, ZHANG Guobin, CHE Xusheng, QIN Qijie. Bioinformation Analysis and Expression Analysis of GME Genes Family in Ascorbic Acid Biosynthesis of Pepper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 71-78. doi: 10.7668/hbnxb.20190723.

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