蓖麻环核苷酸门控离子通道<i>RcCNGC2</i>克隆与盐胁迫下表达分析

doi:10.7668/hbnxb.20190904

华北农学报 ›› 2020, Vol. 35 ›› Issue (4): 79-86. doi: 10.7668/hbnxb.20190904

所属专题： 盐碱胁迫；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

蓖麻环核苷酸门控离子通道RcCNGC2克隆与盐胁迫下表达分析

段琼^1,2,3,4,5, 王晓宇^1,2,3,4,5, 霍红雁^1,2,3,4,5, 何智彪⁶, 张丽雪^1,2,3,4,5, 刘栩铭^1,2,3,4,5, 张洪雨^1,2,3,4,5, 孟迪^1,2,3,4,5, 张继星^1,2,3,4,5

1. 内蒙古民族大学生命科学学院, 内蒙古通辽 028000;
2. 内蒙古民族大学科尔沁植物逆境生物学研究所, 内蒙古通辽 028000;
3. 内蒙古自治区蓖麻育种重点实验室, 内蒙古通辽 028000;
4. 内蒙古自治区蓖麻产业协同创新培育中心, 内蒙古通辽 028000;
5. 内蒙古自治区高校蓖麻产业工程技术研究中心, 内蒙古通辽 028000;
6. 通辽市农业科学研究院, 内蒙古通辽 028000

收稿日期:2020-02-02 出版日期:2020-08-28
通讯作者: 张继星(1971-),男,内蒙古通辽人,教授,博士,主要从事植物抗逆分子生物学研究。
作者简介:段琼(1996-),女,吉林辽源人,在读硕士,主要从事蓖麻抗逆分子生物学研究。
基金资助:
国家自然科学基金项目（31760399；31701467）；内蒙古民族大学自治区科技储备项目（2018NDCB05-3）；内蒙古民族大学研究生科研创新资助项目（NMDSS1954）；自治区高校青年科技英才支持计划项目（NJYT-20-B25）；国家服务特殊需求蒙药学博士点建设专项开放基金项目（MDMYBJ2019008）；内蒙古自治区蓖麻育种重点实验室开放基金项目（MDK2017036；MDK2017037；MDK2019021）

Cloning and Characterization of RcCNGC2 Gene of Ricinus communis L. Cyclic Nucleotide-gated Channel Expression Analysis under Salt Stress

DUAN Qiong^1,2,3,4,5, WANG Xiaoyu^1,2,3,4,5, HUO Hongyan^1,2,3,4,5, HE Zhibiao⁶, ZHANG Lixue^1,2,3,4,5, LIU Xuming^1,2,3,4,5, ZHANG Hongyu^1,2,3,4,5, MENG Di^1,2,3,4,5, ZHANG Jixing^1,2,3,4,5

1. School of Life Sciences, Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Horqin Institute of Plant Stress Biology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
3. Key Laboratory of Castor Breeding, Tongliao 028000, China;
4. Collaborative Innovation and Cultivation Center of Castor Industry, Tongliao 028000, China;
5. Inner Mongolia University Castor Industry Engineering Technology Research Center, Tongliao 028000, China;
6. Tongliao Academy of Agricultural Sciences, Tongliao 028000, China

Received:2020-02-02 Published:2020-08-28

摘要/Abstract

摘要： 为探讨RcCNGC2在蓖麻耐盐中的作用，以通蓖5号叶片为材料，克隆获得环核苷酸门控离子通道RcCNGC2完整编码区序列（CDS），并对该序列进行序列比对、蛋白结构分析，构建多元表达载体，分析了在盐胁迫下蓖麻RcCNGC2基因根、茎、叶组织中6个时间点的表达量变化。结果显示，RcCNGC2 CDS长度为2 148 bp，编码715个氨基酸，蛋白分子量为34.15 ku，等电点为9.96，存在5个跨膜结构域。氨基酸序列一致性分析表明，RcCNGC2与橡胶树一致性最高，达89.15%。qRT-PCR分析结果显示，NaCl处理后RcCNGC2表达量在根中上调且差异显著，在茎、叶中随NaCl处理时间延长而显著减少。综上，RcCNGC2在蓖麻受到盐胁迫时起重要信号传导作用。

关键词: 蓖麻, 环核苷酸门控离子通道, 表达分析, 基因克隆, 生物信息学

Abstract: In order to study the role of RcCNGC2 in the salt tolerance of castor,we cloned and obtained the CDS of RcCNGC2,which was a cyclic nucleotide gated ion channel,from the leaves of Tongbi 5. The CDS of RcCNGC2 was analyzed by bioinformatics,and a multiple expression vector was constructed. The results showed that the CDS length of RcCNGC2 was 2 148 bp,encoding 715 amino acids;the molecular weight of the protein was 34.15 ku; the isoelectric point was 9.96; and there were five transmembrane domains. Amino acid sequence analysis showed that RcCNGC2 had the highest homology with Hevea,up to 89.15%. The results of qRT-PCR showed that the expression of RcCNGC2 was significant difference up-regulated in roots(P<0.05),but decreased significantly in the stem and leaf with the increase of NaCl treatment time. Comprehensively, RcCNGC2 played an important signal transduction role in castor under salt stress.

Key words: Ricinus communis L., Cyclic nucleotide gated channel, Expression analysis, Gene cloning, Bioinformatics

中图分类号:

Q786

段琼, 王晓宇, 霍红雁, 何智彪, 张丽雪, 刘栩铭, 张洪雨, 孟迪, 张继星. 蓖麻环核苷酸门控离子通道RcCNGC2克隆与盐胁迫下表达分析[J]. 华北农学报, 2020, 35(4): 79-86. doi: 10.7668/hbnxb.20190904.

DUAN Qiong, WANG Xiaoyu, HUO Hongyan, HE Zhibiao, ZHANG Lixue, LIU Xuming, ZHANG Hongyu, MENG Di, ZHANG Jixing. Cloning and Characterization of RcCNGC2 Gene of Ricinus communis L. Cyclic Nucleotide-gated Channel Expression Analysis under Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 79-86. doi: 10.7668/hbnxb.20190904.

http://www.hbnxb.net/CN/Y2020/V35/I4/79

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蓖麻环核苷酸门控离子通道RcCNGC2克隆与盐胁迫下表达分析

Cloning and Characterization of RcCNGC2 Gene of Ricinus communis L. Cyclic Nucleotide-gated Channel Expression Analysis under Salt Stress

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蓖麻环核苷酸门控离子通道RcCNGC2克隆与盐胁迫下表达分析

Cloning and Characterization of RcCNGC2 Gene of Ricinus communis L. Cyclic Nucleotide-gated Channel Expression Analysis under Salt Stress

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