华北农学报 ›› 2006, Vol. 21 ›› Issue (S2): 117-119. doi: 10.3321/j.issn:1000-7091.2006.z2.028

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大蒜茎尖脱毒培养及快繁技术研究

张寒霜1, 赵俊丽1, 李伟明1, 付书平1, 王书生2   

  1. 1. 河北省农林科学院棉花研究所, 河北石家庄 050051;
    2. 石家庄市农业局, 河北石家庄 050051
  • 收稿日期:2006-05-22 出版日期:2007-01-01
  • 作者简介:张寒霜(1965- ),女,河北清河人,副研究员,主要从事生物技术研究。
  • 基金资助:
    河北省科技厅项目(03220186D);河北省科院项目(A03-2-02-56);安国县农业局合作项目

Virus Elimination and Rapid Propagation of Garlic by Biotechnology

ZHANG Han-shuang1, ZHAO Jun-li1, LI Wei-ming1, FU Shu-ping1, WANG Shu-sheng2   

  1. 1. Institute of Cotton, Hebei Acadamy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China;
    2. Shijiazhuang Agriculture Bureau, 050051, China
  • Received:2006-05-22 Published:2007-01-01

摘要: 以河北省两个名优地方品种安国大蒜和永年大蒜为试材,用Ms,B5作基本培养基,研究了大蒜茎尖繁殖脱毒快繁技术。结果表明,最佳诱导培养基为B5+0.1mg/L NAA+3mg/L 6_BA,但是,激素、营养物质的添加数量以及培养时期不同品种间有差异;剥离大蒜茎尖带一定数量的鳞片为好,其不仅可提高脱毒苗成活率,而且有利于扩大茎尖繁殖系数。无毒蒜大田扩繁的播种时间对产量有一定影响,且品种间差异较大。

关键词: 大蒜, 茎尖培养, 脱毒, 快繁

Abstract: The virus-eliminating technology with shoot-tip culture and rapid propagation of two local famous elite garlic varieties in Hebei province were studied.The results indicated that the best induce medium for bud propagation was B5+0.1mg/L NAA+3mg/L 6_BA.The numbers of hormone and nutritious and the period cultured were different in different varieties.The garlic shoot-tip with some bud scale is better for culturing.It can increased the survival rate of virus eliminating seedling and propagate rate.The period of virus eliminating garlic be transplanted plant effect the yield and significant different in two varieties.

Key words: Garlic, Biotechnology, Virus eliminition, Rapid propagation

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引用本文

张寒霜, 赵俊丽, 李伟明, 付书平, 王书生. 大蒜茎尖脱毒培养及快繁技术研究[J]. 华北农学报, 2006, 21(S2): 117-119. doi: 10.3321/j.issn:1000-7091.2006.z2.028.

ZHANG Han-shuang, ZHAO Jun-li, LI Wei-ming, FU Shu-ping, WANG Shu-sheng. Virus Elimination and Rapid Propagation of Garlic by Biotechnology[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S2): 117-119. doi: 10.3321/j.issn:1000-7091.2006.z2.028.

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