摘要： 以甜菜8个品系种子、幼苗的茎节、茎尖、叶片(带叶柄)、腋芽等作外植体,对甜菜快繁、植株再生以及再生植株的移栽等技术进行了研究。结果表明,不同外植体在 MS+6-BA 0.8～1.5mg/L+NAA 0.2～0.3mg/L 培养基上,诱导效果最佳,而且幼苗生长健壮。生根培养在 MS+NAA 1.0～1.5mg/L 或 MS+NAA 1.0～1.2mg/L+IBA0.2mg/L 上,生根率可达85%以上,且根系发达,再生植株的移栽成活率达90%以上。建立了甜菜组培快繁及植株再生的技术体系,同时该再生体系可作为基因转化的受体系统。

关键词: 甜菜, 组织培养, 快繁, 植株再生

Abstract: Four kinds of explants including seeds, stem segments of seedling, shoot tips with petioles,leaves and axillary bud of eight sweet beets strains were used to study the rapid-multiplication, theregeneration of plantlets, plat transplanting of plantlets in vitro. The results showed: MS+6-BA 0.8-1.Smg/L+NAA 0.2–0.3mg/L is the best medium for regenerated plantlets induction to all kinds of explants, and the plantlet grows robust. Using MS+NAA 1.0–1.5mg/L or MS+NAA 1.0 l.2mg/L+IBA 0.2mg/L as rooting culture media, the rooting rate will be more than 85%, and root is developed, the survival rate of regenerated pla}itlets reaches more than 90%. A series of plantlet regeneration and rapid-multiplication in vitro system of sweet beet has been set up, which can also be used as receptor system for gene transfer.

Key words: subarbeet, Tissue culture, Plantlet regeneration, Rapid-Multiplication

中图分类号: 

• S566.3