Combinations of Phosphate-Solubilizing Bacteria Promote Maize Growth and Phosphorus Uptake

doi:10.7668/hbnxb.20196388

Abstract

Abstract:

To investigate the phosphate-solubilizing effects of different combinations of phosphate-solubilizing bacteria(PSB)and their impact on maize growth and phosphorus uptake,a pot experiment was conducted using three bacterial strains with distinct taxonomic classifications and phosphate-solubilizing capacities:Pseudomonas frederiksbergensis P4-5,Arthrobacter oryzae P5-41,and Bacillus aryabhattai P3-50.A non-inoculated treatment served as the blank control(CK),and the experimental treatments included:single inoculation with P4-5(Pf),single inoculation with P5-41(Ao),single inoculation with P3-50(Ba),dual inoculation with P4-5 and P5-41(Pf×Ao),dual inoculation with P4-5 and P3-50(Pf×Ba),dual inoculation with P5-41 and P3-50(Ao×Ba),and combined inoculation with P4-5,P5-41,and P3-50(Pf×Ao×Ba).The effects of these inoculation treatments on maize root morphology,shoot growth,phosphorus uptake,and indole-3-acetic acid(IAA)secretion capacity by the bacterial strains were analyzed.The results indicated that,compared to the CK,all PSB inoculations significantly increased maize stem diameter,leaf SPAD value,shoot biomass,and phosphorus uptake.Furthermore,they also significantly enhanced rhizosphere alkaline phosphatase activity(except for single inoculation with Pf and Ba,respectively)and reduced soil available phosphorus content(except for single inoculation with Ba).Among these treatments,the combined inoculation of Pf×Ao×Ba exhibited the best overall performance.This treatment secreted high concentrations of IAA,optimized maize root morphology(increasing root length,volume,surface area,and number of root tips),and significantly increased rhizosphere alkaline phosphatase activity,thereby promoting the mineralization of soil organic phosphorus and consequently enhancing maize growth and phosphorus uptake.In conclusion,the combined inoculation of Pf×Ao×Ba significantly promoted maize growth and improved phosphorus nutrition through a dual mechanism:optimizing root morphology by increasing root length,volume,surface area,and root tip number through IAA secretion,and effectively mineralizing soil organic phosphorus by enhancing alkaline phosphatase activity.

Key words: Maize, Phosphate-solubilizing bacteria(PSB), Combined inoculation, Root morphology, Alkaline phosphatase activity, Phosphate uptake

CLC Number:

SUN Yaqin, CHENG Yanan, LI Hang, LIU Xing, ZHANG Ying, WANG Fei. Combinations of Phosphate-Solubilizing Bacteria Promote Maize Growth and Phosphorus Uptake[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(2): 138-145. doi: 10.7668/hbnxb.20196388.

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References 40

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处理 Treatment	株高/cm Plant height	茎粗/cm Stem diameter	叶面积/cm² Leaf area	SPAD值 SPAD value
CK	33.40±0.17bc	7.43±0.04c	8 519.07±17.61ab	27.27±0.65b
Pf	45.70±2.98a	8.57±0.24ab	9 270.10±167.03a	32.67±1.27a
Ao	42.15±0.66a	8.42±0.16ab	8 356.90±576.22ab	32.27±0.64a
Ba	33.25±1.24bc	8.65±0.34ab	9 008.50±313.20a	33.07±1.56a
Pf×Ao	30.07±0.43c	8.88±0.13a	7 675.88±537.37b	34.80±1.99a
Pf×Ba	36.03±0.47b	8.15±0.23b	8 242.95±149.17ab	32.50±0.06a
Ao×Ba	37.53±2.07b	8.50±0.19ab	8 205.94±96.89ab	34.97±1.14a
Pf×Ao×Ba	34.85±1.53bc	9.08±0.20a	9 318.51±427.73a	32.50±1.21a

处理 Treatment	株高/cm Plant height	茎粗/cm Stem diameter	叶面积/cm² Leaf area	SPAD值 SPAD value
CK	33.40±0.17bc	7.43±0.04c	8 519.07±17.61ab	27.27±0.65b
Pf	45.70±2.98a	8.57±0.24ab	9 270.10±167.03a	32.67±1.27a
Ao	42.15±0.66a	8.42±0.16ab	8 356.90±576.22ab	32.27±0.64a
Ba	33.25±1.24bc	8.65±0.34ab	9 008.50±313.20a	33.07±1.56a
Pf×Ao	30.07±0.43c	8.88±0.13a	7 675.88±537.37b	34.80±1.99a
Pf×Ba	36.03±0.47b	8.15±0.23b	8 242.95±149.17ab	32.50±0.06a
Ao×Ba	37.53±2.07b	8.50±0.19ab	8 205.94±96.89ab	34.97±1.14a
Pf×Ao×Ba	34.85±1.53bc	9.08±0.20a	9 318.51±427.73a	32.50±1.21a

处理 Treatment	根系长度/cm Root length	根尖数/个 Root tips number	根系体积/cm³ Root volume	根系表面积/cm² Root surface area
CK	937.62±5.88c	1 908.00±23.25c	5.73±0.05bc	158.23±1.13bcd
Pf	909.84±39.92c	2 189.67±149.82abc	4.47±0.09cd	143.94±4.82cd
Ao	916.27±77.36c	2 226.33±88.44abc	3.86±0.37d	136.38±15.41d
Ba	908.19±31.99c	1 811.00±84.01c	6.52±0.73ab	183.32±13.68ab
Pf×Ao	1 115.61±20.53bc	1 921.67±63.85c	4.47±0.13cd	152.08±11.28bcd
Pf×Ba	1 068.50±74.09bc	2 125.33±83.65bc	6.01±0.82b	177.02±8.11abc
Ao×Ba	1 264.38±48.56ab	2 496.67±148.85ab	7.15±0.56ab	207.94±15.32a
Pf×Ao×Ba	1 402.51±139.81a	2 631.67±285.37a	7.69±0.14a	211.85±11.07a

处理 Treatment	根系长度/cm Root length	根尖数/个 Root tips number	根系体积/cm³ Root volume	根系表面积/cm² Root surface area
CK	937.62±5.88c	1 908.00±23.25c	5.73±0.05bc	158.23±1.13bcd
Pf	909.84±39.92c	2 189.67±149.82abc	4.47±0.09cd	143.94±4.82cd
Ao	916.27±77.36c	2 226.33±88.44abc	3.86±0.37d	136.38±15.41d
Ba	908.19±31.99c	1 811.00±84.01c	6.52±0.73ab	183.32±13.68ab
Pf×Ao	1 115.61±20.53bc	1 921.67±63.85c	4.47±0.13cd	152.08±11.28bcd
Pf×Ba	1 068.50±74.09bc	2 125.33±83.65bc	6.01±0.82b	177.02±8.11abc
Ao×Ba	1 264.38±48.56ab	2 496.67±148.85ab	7.15±0.56ab	207.94±15.32a
Pf×Ao×Ba	1 402.51±139.81a	2 631.67±285.37a	7.69±0.14a	211.85±11.07a

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