Exploring Aluminum Tolerance Genes in Brassica napus Based on BSA-seq

doi:10.7668/hbnxb.20196292

Abstract

Abstract:

In recent years,soil acidification and aluminum ion toxicity in southern cultivated land have intensified.To explore the gene loci related to aluminum tolerance in rapeseed,the F₂ population obtained by crossing the aluminum tolerant variety R248 with the aluminum sensitive variety S120 was used as the experimental material.The extreme mixed pool of aluminum toxicity tolerance was constructed by phenotypic identification,and QTL localization analysis was performed by BSA-seq.The phenotype identification results showed that the aluminum toxicity tolerance of the F₂ population exhibited a continuous normal distribution.After sequencing,mutation detection,and association analysis,a total of 4 intervals related to aluminum toxicity tolerance were obtained,distributed on chromosomes A07,C02,C03,and C08,with a total length of 0.73 Mb and 98 genes.Based on the enrichment analysis results and gene annotation information,it is predicted that BnaA07g35600D,BnaA07g35660D,BnaA07g35700D,BnaA07g3570D, and BnaC03g39670D will participate in the synthesis and transportation of aluminum tolerance hormones in rapeseed,BnaC03g39840D participates in the synthesis of cell wall pectinesterase,BnaC03g39750D and BnaC08g20580D were transcription factors with MYB structure,BnaC03g39850D and BnaC03g39980D were involved in amino acid metabolism.These genes are highly correlated with aluminum tolerance in rapeseed,laying the foundation for the cloning and functional research of aluminum tolerance genes in the next step.

Key words: Brassica napus L., Aluminum toxicit, BSA-seq, Candidate gene

CLC Number:

S565.4

ZHANG Meiwei, CHEN Ming, HAN Depeng, LI Yazhen, XIAO Xiaojun, XIONG Wen, HUANG Tianbao, CHENG Yewei, ZHANG Chen, ZHOU Ying, ZHENG Wei. Exploring Aluminum Tolerance Genes in Brassica napus Based on BSA-seq[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 30-38. doi: 10.7668/hbnxb.20196292.

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References 40

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样品 Sample	原始碱基数/bp Raw bases	高质量碱基数/bp Clean bases	Q20/%	Q30/%	GC 含量/% GC content
R248	11 865 770 100	11 608 838 100	98.66	96.34	37.59
S120	11 221 807 800	10 997 508 300	98.73	96.55	37.41
BnaRF2	37 068 239 100	36 162 695 100	97.31	92.86	37.65
BnaSF2	30 531 714 600	29 793 675 300	98.11	94.72	37.70

样品 Sample	原始碱基数/bp Raw bases	高质量碱基数/bp Clean bases	Q20/%	Q30/%	GC 含量/% GC content
R248	11 865 770 100	11 608 838 100	98.66	96.34	37.59
S120	11 221 807 800	10 997 508 300	98.73	96.55	37.41
BnaRF2	37 068 239 100	36 162 695 100	97.31	92.86	37.65
BnaSF2	30 531 714 600	29 793 675 300	98.11	94.72	37.70

样品 Sample	总reads数 Total reads	比对reads数 Mapped reads	比对率/% Mapping rate	深度/× Depth	覆盖度/% Coverage
R248	77 392 254	76 463 182	98.80	12.98	92.36
S120	73 316 722	72 259 034	98.56	12.20	91.09
BnaRF2	241 084 634	237 490 700	98.51	37.33	96.70
BnaSF2	198 624 502	195 864 711	98.61	34.36	96.49

样品 Sample	总reads数 Total reads	比对reads数 Mapped reads	比对率/% Mapping rate	深度/× Depth	覆盖度/% Coverage
R248	77 392 254	76 463 182	98.80	12.98	92.36
S120	73 316 722	72 259 034	98.56	12.20	91.09
BnaRF2	241 084 634	237 490 700	98.51	37.33	96.70
BnaSF2	198 624 502	195 864 711	98.61	34.36	96.49

类别 Category	父本 R248	母本 S120	耐铝混池 BnaRF2	铝敏感混池 BnaSF2
外显子 Exonic	475 614	568 540	845 322	842 275
外显子终止位点获得 Exonic stop gain	2 758	3 305	4 876	4 878
外显子终止位点缺失 Exonic stop loss	670	789	1 201	1 190
外显子同义突变 Exonic synonymous	277 106	331 708	493 820	491 814
外显子非同义突变 Exonic non-synonymous	195 080	232 738	345 425	344 393
内含子 Intronic	437 756	518 381	786 483	781 894
剪接 Splicing	1 880	2 359	3 369	3 340
上游 Upstream	307 434	367 416	545 231	544 291
下游 Downstream	254 490	303 816	453 128	451 073
上游/下游 Upstream/Downstream	63 705	76 221	113 439	113 706
基因间区 Intergenic	1 896 598	2 170 181	3 252 827	3 235 603
3'非翻译区 3'UTR	45 513	55 364	81 530	81 325
5'非翻译区 5'UTR	30 910	36 788	54 621	54 545
总计 Total	3 513 900	4 099 066	6 135 950	6 108 052

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