Identification and Pathogenicity Evaluation of Pepper Root Rot Pathogens in Xigaze Area,Tibet

doi:10.7668/hbnxb.20195736

Abstract

Abstract:

To detect and identify the pathogenic species of pepper root rot in Xigaze,Tibet,16 samples of chili root susceptible plants were collected from four districts,namely,Pengcang village,Bazha township,Bailang county;Pankong village,Nerixiong township,Sangzhuzi District;Deji village,Dazi township,Jiangzi county;and Yuze village,Lazi township,Lazi county,Tibet,from July to September 2024.A total of 25 strains of pathogenic fungi were isolated and purified by conventional tissue isolation method,and 3 types of fungal isolates were grouped into LJ1,LJ2 and LJ3.Using molecular biology and phylogenetic analysis,LJ1 was identified as Fusarium solani,LJ2 as Rhizoctonia solani AG 4 HG-Ⅰ,and LJ3 as Fusarium oxysporum.The pathogenicity of the fungi was evaluated by the seedling prick inoculation method.All three types of fungal isolates were able to infect pepper seedlings causing root rot,and F.oxysporum had the strongest pathogenicity.There were differences in the dominant fungal pathogens of pepper root rot across Xigaze,with F.oxysporum being the dominant pathogen with 52.0% isolation frequency, F.solani with 32.0% isolation frequency and R.solani with 16.0% isolation frequency.The root rot of pepper in the Xigaze region of Tibet was caused by a complex infection of F.oxysporum,F.solani, and R.solani AG 4 HG-Ⅰ,both F.solani and R.solani AG 4 HG-Ⅰ were isolated and identified for the first time in Tibet.

Key words: Pepper root rot, Fusarium solani, Fusarium oxysporum, Rhizoctonia solani AG4HG-Ⅰ, Pathogenicity

CLC Number:

LI Xin, Gesang Wangmu, WANG Guanjie, XIE Xuewen, SHI Yanxia, LI Lei, FAN Tengfei, LI Baoju, CHAI Ali. Identification and Pathogenicity Evaluation of Pepper Root Rot Pathogens in Xigaze Area,Tibet[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 194-201. doi: 10.7668/hbnxb.20195736.

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Figures/Tables 9

Fig.1 Field symptoms of pepper root rot A.Diseased pepper plant;B.Surface symptoms at the base of the diseased stem;C.Internal symptoms at the base of the diseased stem.

Tab.1 Isolation of pepper root rot strains in different regions of Xigaze

采集地 Collection location	菌株 Strains
采集地 Collection location	LJ1	LJ2	LJ3
白朗县巴扎乡彭仓村 Pengcang village, Bazha township, Bailang county	4	2	5
桑珠孜区聂日雄乡盘孔村 Pankong village, Nerixiong township, Sangzhuzi District	2	1	3
江孜县达孜乡德吉村 Deji village, Dazi township, Jiangzi county	0	1	2
拉孜县拉孜镇玉哲村 Yuzhe village, Lazi township, Lazi county	2	0	3
合计Total	8	4	13

Fig.2 Morphological characteristics of Fusarium solani A.Colony morphology;B.Macroconidia and sporidochium;C.Microconidia.

Fig.3 Morphological characteristics of Rhizoctonia solani A.Colony morphology;B.Mycelial morphology;C.Mycelial expansion.

Fig.4 Morphological characteristics of Fusarium oxysporum A.Colony morphology;B.Macroconidia;C.Microconidia;D.Chlamydospores.

Fig.5 Phylogenetic analysis of Fusarium solani and Fusarium oxysporum based on ITS and TEF-1α genes

Fig.6 Phylogenetic analysis of Rhizoctonia solani based on ITS and TEF-1α genes

Fig.7 Symptoms observed in pathogenicity tests at 7 days after inoculation A.Overall symptoms of healthy plants and pepper seedlings inoculated with pathogen at 7 days;B.Root morphology of healthy seedlings;C.Symptoms of the root system of seedlings inoculated with pathogen at 7 days.

Fig.8 Distribution of pathogens of pepper root rot in the Xigaze Region

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