Effects of Streptomyces TOR3209 Fermentation Liquid VOCs on Tobacco Growth and Development

doi:10.7668/hbnxb.20196009

Abstract

Abstract:

The biological mechanism of Streptomyces TOR3209 volatile organic compounds(VOCs)promoting tobacco growth and development was studied.The growth-promoting properties of VOCs in the fermentation broth were identified by two separate plates,and the VOCs components were analyzed by solid phase microextraction and gas chromatography-mass spectrometry.VOCs of Streptomyces TOR3209 at different concentrations could promote the growth of tobacco,and the effect of adding 5 mL(6×10⁷ CFU/mL)strain fermentation liquid was the most significant in the treatment group,which increased 238% compared with the control group.A total of 32 substances were extracted from the VOCs of Streptomyces TOR3209 fermentation broth,and the growth-promoting function of dinbutyl adipate was verified.The results showed that dinbutyl adipate of different mass concentrations could promote the growth of tobacco,and the growth promoting effect of 1 mol/mL was the most significant.Through transcriptome analysis,it was found that many genes such as laccase were up-regulated in the tobacco treated group compared with the control group.VOCs of Streptomyces TOR3209 had a significant growth promoting effect on tobacco,and dinbutyl adipate regulated several growth promoting genes in tobacco.This study clarified the mechanism of the VOCs of Streptomyces TOR3209 regulating tobacco growth and development,and provided a theoretical basis for the development of efficient and environmentally friendly microbial production promotion products and the application evaluation.

Key words: Volatile organic compounds, Streptomyces, Transcriptome, Tobacco

CLC Number:

S572
Q946

ZHANG Mengyi, HE Yuxi, PENG Jieli, ZHAO Linlin, ZHANG Xiumin, YANG Taixin, HU Dong. Effects of Streptomyces TOR3209 Fermentation Liquid VOCs on Tobacco Growth and Development[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 277-284. doi: 10.7668/hbnxb.20196009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20196009

http://www.hbnxb.net/EN/Y2025/V40/IS1/277

Figures/Tables 8

References 40

[1]	李俊, 姜昕, 马鸣超, 关大伟, 曹凤明, 李力. 我国微生物肥料产业需求与技术创新[J]. 中国土壤与肥料, 2019(2):1-5.doi:10.11838/sfsc.1673-6257.19029.
	Li J, Jiang X, Ma M C, Guan D W, Cao F M, Li L. Development demand and technical innovation for bio-fertilizer industry in China[J]. Soil and Fertilizer Sciences in China, 2019(2):1-5.
[2]	Kiprotich K, Muema E, Wekesa C, Ndombi T, Muoma J, Omayio D, Ochieno D, Motsi H, Mncedi S, Tarus J. Unveiling the roles,mechanisms and prospects of soil microbial communities in sustainable agriculture[J]. Discover Soil, 2025, 2(1):10.doi:10.1007/s44378-025-00037-4.
[3]	Xie W, Ding C Q, Hu H T, Dong G J, Zhang G H, Qian Q, Ren D Y. Molecular events of rice AP2/ERF transcription factors[J]. International Journal of Molecular Sciences, 2022, 23(19):12013.doi:10.3390/ijms231912013. URL
[4]	张瑞福, 沈其荣. 微生物肥料新型功能作用机理与根际定殖增强策略[J]. 微生物学杂志, 2024, 44(1):1-11.doi:10.3969/j.issn.1005-7021.2024.01.001.
	Zhang R F, Shen Q R. Mechanisms of the microbial fertilizer's novel functions and the strategies to enhance its root colonization[J]. Journal of Microbiology, 2024, 44(1):1-11.
[5]	张福锁, 黄成东, 申建波, 危常州, 马文奇, 吕阳, 鲁振亚, 朱齐超, 石孝均, 侯翠红, 马航. 绿色智能肥料:矿产资源养分全量利用的创新思路与产业化途径[J]. 土壤学报, 2023, 60(5):1203-1212.doi:10.11766/trxb202305190196.
	Zhang F S, Huang C D, Shen J B, Wei C Z, Ma W Q, Lyu Y, Lu Z Y, Zhu Q C, Shi X J, Hou C H, Ma H. Green intelligent fertilizer:new insight into making full use of mineral nutrient resources and industrial approach[J]. Acta Pedologica Sinica, 2023, 60(5):1203-1212.
[6]	杨腾峰, 闫建培, 侯星羽, 沈洪飞, 段佳琪, 任杰, 曾洪梅. 苜蓿链霉菌TX21抑菌作用确定及促生物质的鉴定[J]. 中国生物防治学报, 2024, 40(2):359-369.doi:10.16409/j.cnki.2095-039x.2024.02.010.
	Yang T F, Yan J P, Hou X Y, Shen H F, Duan J Q, Ren J, Zeng H M. Inhibition of Streptomyces alfalfa TX21 and identification of biotropic substances[J]. Chinese Journal of Biological Control, 2024, 40(2):359-369.
[7]	Wang T H, Ahmad S, Yang L, Yan X N, Zhang Y F, Zhang S J, Wang L Y, Luo Y P. Preparation,biocontrol activity and growth promotion of biofertilizer containing Streptomyces aureoverticillatus HN6[J]. Frontiers in Plant Science, 2022, 13:1090689.doi:10.3389/fpls.2022.1090689.
[8]	Hu D, Li S H, Li Y, Peng J L, Wei X Y, Ma J, Zhang C M, Jia N, Wang E T, Wang Z W. Streptomyces sp.strain TOR3209:a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community[J]. Scientific Reports, 2020,10:20132.doi:10.1038/s41598-020-76887-5.
[9]	Ma J, Peng J L, Tian S, He Y X, Zhang C M, Jia N, Wang E T, Wang Z W, Hu D. Streptomyces sp.TOR3209 alleviates cold stress in tomato plants[J]. New Zealand Journal of Crop and Horticultural Science, 2023, 51(4):662-682.doi:10.1080/01140671.2022.2066141. URL
[10]	Rao Y X, Zeng L Z, Jiang H, Mei L, Wang Y J. Trichoderma atroviride LZ42 releases volatile organic compounds promoting plant growth and suppressing Fusarium wilt disease in tomato seedlings[J]. BMC Microbiology, 2022, 22(1):88.doi:10.1186/s12866-022-02511-3.
[11]	Jiang L M, Lee M H, Kim C Y, Kim S W, Kim P I, Min S R, Lee J. Plant growth promotion by two volatile organic compounds emitted from the fungus Cladosporium halotolerans NGPF1[J]. Frontiers in Plant Science, 2021, 12:794349.doi:10.3389/fpls.2021.794349.
[12]	Joo J H, Hussein K A. Biological control and plant growth promotion properties of volatile organic compound-producing antagonistic Trichoderma spp.[J]. Frontiers in Plant Science, 2022, 13:897668.doi:10.3389/fpls.2022.897668.
[13]	He Y X, Guo W Y, Peng J L, et al. Volatile organic compounds of Streptomyces sp.TOR3209 stimulated tobacco growth by up-regulating the expression of genes related to plant growth and development[J]. Frontiers in Microbiology, 2022, 13:891245.doi:10.3389/fmicb.2022.891245.
[14]	Li Y C, Shao J H, Xie Y M, et a. Volatile compounds from beneficial rhizobacteria Bacillus spp.promote periodic lateral root development in Arabidopsis[J]. Plant,Cell & Environment, 2021, 44(5):1663-1678.doi:10.1111/pce.14021. URL
[15]	Wang K, Lin Z Y, Dou J, Jiang M G, Shen N K, Feng J. Identification and surveys of promoting plant growth VOCs from biocontrol bacteria Paenibacillus peoriae GXUN15128[J]. Microbiology Spectrum, 2023, 11(3):e04346-e04322.doi:10.1128/spectrum.04346-22.
[16]	Macías-Rubalcava M L, Hernández-Bautista B E, Oropeza F, Duarte G, González M C, Glenn A E, Hanlin R T, Anaya A L. Allelochemical effects of volatile compounds and organic extracts from Muscodor yucatanensis,a tropical endophytic fungus from Bursera simaruba[J]. Journal of Chemical Ecology, 2010, 36(10):1122-1131.doi:10.1007/s10886-010-9848-5. pmid: 20809145
[17]	Hu D, Li X Z, Chang Y L, He H, Zhang C M, Jia N, Li H T, Wang Z W. Genome sequence of Streptomyces sp.strain TOR3209,a rhizosphere microecology regulator isolated from tomato rhizosphere[J]. Journal of Bacteriology, 2012, 194(6):1627.doi:10.1128/jb.06684-11. URL
[18]	Janusz G, Pawlik A, S'widerska-Burek U, Polak J, Sulej J, Jarosz-Wilkołazka A, Paszczyński A. Laccase properties,physiological functions,and evolution[J]. International Journal of Molecular Sciences, 2020, 21(3):966.doi:10.3390/ijms21030966. URL
[19]	Bai Y S, Ali S, Liu S, Zhou J J, Tang Y L. Characterization of plant laccase genes and their functions[J]. Gene, 2023, 852:147060.doi:10.1016/j.gene.2022.147060.
[20]	Ding Y J, Zhang X X, Li J L, Wang R Y, Chen J, Kong L N, Li X, Yang Z M, Zhuang L L. Transcriptome-based weighted gene co-expression network analysis reveals the photosynthesis pathway and hub genes involved in promoting tiller growth under repeated drought rewatering cycles in perennial ryegrass[J]. Plants, 2024, 13(6):854.doi:10.3390/plants13060854. URL
[21]	Sharma P, Gayen D. Plant protease as regulator and signaling molecule for enhancing environmental stress-tolerance[J]. Plant Cell Reports, 2021, 40(11):2081-2095.doi:10.1007/s00299-021-02739-9.
[22]	Mao Y B, Xue X Y, Tao X Y, Yang C Q, Wang L J, Chen X Y. Cysteine protease enhances plant-mediated bollworm RNA interference[J]. Plant Molecular Biology, 2013, 83(1):119-129.doi:10.1007/s11103-013-0030-7. URL
[23]	Li Y, Wang K, Xie H, Wang D W, Xu C L, Huang X, Wu W J, Li D L. Cathepsin B cysteine proteinase is essential for the development and pathogenesis of the plant parasitic nematode Radopholus similis[J]. International Journal of Biological Sciences, 2015, 11(9):1073-1087.doi:10.7150/ijbs.12065. URL
[24]	Rea P A. Plant ATP-binding cassette transporters[J]. Annual Review of Plant Biology, 2007, 58:347-375.doi:10.1146/annurev.arplant.57.032905.105406. pmid: 17263663
[25]	Zhang H S, Jing W, Zheng J M, Jin Y Y, Wu D, Cao C J, Dong Y M, Shi X Y, Zhang W H. The ATP-binding cassette transporter OsPDR1 regulates plant growth and pathogen resistance by affecting jasmonates biosynthesis in rice[J]. Plant Science, 2020, 298:110582.doi:10.1016/j.plantsci.2020.110582.
[26]	Ortiz A, Sansinenea E. Phenylpropanoid derivatives and their role in plants' health and as antimicrobials[J]. Current Microbiology, 2023, 80(12):380.doi:10.1007/s00284-023-03502-x. pmid: 37864088
[27]	Liu Y M, Cao L, Wu X, Wang S, Zhang P M, Li M L, Jiang J H, Ding X D, Cao X Y. Functional characterization of wild soybean(Glycine soja)GsSnRK1.1 protein kinase in plant resistance to abiotic stresses[J]. Journal of Plant Physiology, 2023, 280:153881.doi:10.1016/j.jplph.2022.153881.
[28]	Jia H T, Li M F, Li W Y, et al. A serine/threonine protein kinase encoding gene kernel number per row6 regulates maize grain yield[J]. Nature Communications, 2020,11:988.doi:10.1038/s41467-020-14746-7.
[29]	Huang X X, Zhao S M, Zhang Y Y, Li Y J, Shen H N, Li X G, Hou B K. A novel UDP-glycosyltransferase 91C1 confers specific herbicide resistance through detoxification reaction in Arabidopsis[J]. Plant Physiology and Biochemistry, 2021, 159:226-233.doi:10.1016/j.plaphy.2020.12.026. URL
[30]	Dong N Q, Lin H X. Contribution of phenylpropanoid metabolism to plant development and plant environment interactions[J]. Journal of Integrative Plant Biology, 2021, 63(1):180-209.doi:10.1111/jipb.13054. URL
[31]	Zakrzewska-Placzek M, Golisz-Mocydlarz A, Krzyszton M, Piotrowska J, Lichocka M, Kufel J. The nucleolar protein NOL12 is required for processing of large ribosomal subunit rRNA precursors in Arabidopsis[J]. BMC Plant Biology, 2023, 23(1):538.doi:10.1186/s12870-023-04561-9. pmid: 37919659
[32]	Manna M, Rengasamy B, Sinha A K. Revisiting the role of MAPK signalling pathway in plants and its manipulation for crop improvement[J]. Plant,Cell & Environment, 2023, 46(8):2277-2295.doi:10.1111/pce.14606. URL
[33]	Zuo Z F, Lee H Y, Kang H G. Basic helix-loop-helix transcription factors:regulators for plant growth development and abiotic stress responses[J]. International Journal of Molecular Sciences, 2023, 24(2):1419.doi:10.3390/ijms24021419. URL
[34]	贾雨彤. 拟南芥转录因子bHLH146的功能研究[D]. 长春: 吉林大学, 2022.doi:10.27162/d.cnki.gjlin.2022.006447.
	Jia Y T. Functional study of Arabidopsis transcription factor bHLH146[D]. Changchun: Jilin University, 2022.
[35]	郭晓江. bHLH转录因子PGS1调控Fl3影响谷物种子发育和产量[D]. 雅安: 四川农业大学, 2022.doi:10.27345/d.cnki.gsnyu.2022.000588.
	Guo X J. The PGS1 basic helix-loop-helix(bHLH)protein regulates Fl3 to impact seed growth and grain yield in cereals[D]. Yaan: Sichuan Agricultural University, 2022.
[36]	Wang Y J, Zhou H Y, He Y R, Shen X P, Lin S E, Huang L. MYB transcription factors and their roles in the male reproductive development of flowering plants[J]. Plant Science, 2023, 335:111811.doi:10.1016/j.plantsci.2023.111811.
[37]	Baldoni E, Genga A, Cominelli E. Plant MYB transcription factors:their role in drought response mechanisms[J]. International Journal of Molecular Sciences, 2015, 16(7):15811-15851.doi:10.3390/ijms160715811. pmid: 26184177
[38]	Li C X, Yu W J, Xu J R, Lu X F, Liu Y Z. Anthocyanin biosynthesis induced by MYB transcription factors in plants[J]. International Journal of Molecular Sciences, 2022, 23(19):11701.doi:10.3390/ijms231911701. URL
[39]	Feller A, Machemer K, Braun E L, Grotewold E. Evolutionary and comparative analysis of MYB and bHLH plant transcription factors[J]. The Plant Journal, 2011, 66(1):94-116.doi:10.1111/j.1365-313x.2010.04459.x.
[40]	Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice[J]. Plant Physiology, 2006, 140(2):411-432.doi:10.1104/pp.105.073783. URL

序号 Number	化合物名称 Name of compound	CAS 编号 CAS number	相对含量/% Relative content
1	己二酸二丁酯	000105-99-7	15.98
2	棕榈酸	000057-10-3	11.49
3	2,4-二叔丁基酚	000096-76-4	5.41
4	十五烷酸	001002-84-2	4.95
5	六噻烷	013798-23-7	3.82
6	1 19-二十碳二烯	014811-95-1	3.07
7	肉豆蔻酸	000544-63-8	2.61
8	(Z)-9-十六碳烯醛	056219-04-6	2.18
9	十七烷酸	000506-12-7	2.30
10	α-panasinsanene	056633-28-4	1.27
11	十六醛	000629-80-1	1.05
12	月桂酸	000143-07-7	0.26
13	二十四烷醇	000506-51-4	0.43
14	正十七烷	000629-78-7	0.82
15	9-Eicosene, (9E)-	074685-29-3	0.92
16	环十五烷	000295-48-7	0.38
17	植酮	000502-69-2	0.49
18	环十四烷	000295-17-0	0.47
19	2,4,6-Trimethyl-undecanoic	055955-76-5	0.59
20	2-十四酮	002345-27-9	0.47
21	α-eudesmol	000473-16-5	0.45
22	十七烷酮	002922-51-2	0.29
23	正十七烷	000629-78-7	1.05
24	2,2,4,4-tetramethyl	097455-13-5	0.27
25	4-氨基-3-甲基苯酚	002835-99-6	0.26
26	2-羟基环十五烷酮	004727-18-8	0.33
27	十六酸乙酯	000628-97-7	0.61
28	十三酸乙酯	028267-29-0	0.47
29	环十六烷	000295-65-8	0.57
30	(3E)-3-十八碳烯	007206-19-1	0.29
31	2-环己烯-1-醇	000822-67-3	0.39
32	十七烷酸乙酯	014010-23-2	0.26

序号 Number	化合物名称 Name of compound	CAS 编号 CAS number	相对含量/% Relative content
1	己二酸二丁酯	000105-99-7	15.98
2	棕榈酸	000057-10-3	11.49
3	2,4-二叔丁基酚	000096-76-4	5.41
4	十五烷酸	001002-84-2	4.95
5	六噻烷	013798-23-7	3.82
6	1 19-二十碳二烯	014811-95-1	3.07
7	肉豆蔻酸	000544-63-8	2.61
8	(Z)-9-十六碳烯醛	056219-04-6	2.18
9	十七烷酸	000506-12-7	2.30
10	α-panasinsanene	056633-28-4	1.27
11	十六醛	000629-80-1	1.05
12	月桂酸	000143-07-7	0.26
13	二十四烷醇	000506-51-4	0.43
14	正十七烷	000629-78-7	0.82
15	9-Eicosene, (9E)-	074685-29-3	0.92
16	环十五烷	000295-48-7	0.38
17	植酮	000502-69-2	0.49
18	环十四烷	000295-17-0	0.47
19	2,4,6-Trimethyl-undecanoic	055955-76-5	0.59
20	2-十四酮	002345-27-9	0.47
21	α-eudesmol	000473-16-5	0.45
22	十七烷酮	002922-51-2	0.29
23	正十七烷	000629-78-7	1.05
24	2,2,4,4-tetramethyl	097455-13-5	0.27
25	4-氨基-3-甲基苯酚	002835-99-6	0.26
26	2-羟基环十五烷酮	004727-18-8	0.33
27	十六酸乙酯	000628-97-7	0.61
28	十三酸乙酯	028267-29-0	0.47
29	环十六烷	000295-65-8	0.57
30	(3E)-3-十八碳烯	007206-19-1	0.29
31	2-环己烯-1-醇	000822-67-3	0.39
32	十七烷酸乙酯	014010-23-2	0.26

己二酸二正丁酯 Dibutyl adipate	鲜质量/g Fresh weight
CK	0.021±0.005b
M1	0.028±0.010b
M2	0.022±0.015b
M3	0.049±0.022a

己二酸二正丁酯 Dibutyl adipate	鲜质量/g Fresh weight
CK	0.021±0.005b
M1	0.028±0.010b
M2	0.022±0.015b
M3	0.049±0.022a

Please choose a citation manager

Content to export