Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality

doi:10.7668/hbnxb.20195799

Abstract

Abstract:

OsAAP8 overexpressed transgenic lines were constructed based on the japonica rice variety Zhonghua 11.By detecting and analyzing its agronomic and quality traits,it explored the effects of OsAAP8 overexpression on rice growth and development,as well as rice quality,to provide theoretical basis for molecular design breeding by utilizing OsAAP8 gene to improve rice quality and yield.The results showed that compared with the transgenic negative plants,OsAAP8 overexpressed transgenic positive plants had significantly reduced plant height,tiller number,and single plant yield.The quality trait testing results showed that the content of glutamic acid,threonine,essential amino acids,total amino acids,protein,amylose,gel consistency,and gelatinization temperature in OsAAP8 overexpressed transgenic positive rice were significantly increased.The content of total starch and brown rice rate did not change significantly.The content of free fatty acids,taste value,polished rice rate,and whole polished rice rate were significantly decreased.The observation results of optical microscope and scanning electron microscope showed that the chalkiness rate and chalkiness degree of OsAAP8 overexpressed transgenic positive rice were significantly increased,but the chalkiness area did not change significantly,and the shape and arrangement of starch granules did not change.The grain size detection results showed no significant changes in grain length,width,and thickness of OsAAP8 overexpressed transgenic positive rice.The above results indicate that overexpression of OsAAP8 is not conducive to the growth and development of rice,but can significantly improve the nutritional quality of rice,which provides important information for the cultivation of new high-quality rice varieties.

Key words: Rice, Amino acid permease, OsAAP8, Rice quality, Overexpression

CLC Number:

S511稻

PENG Bo, LIU Yan, XU Ruxin, XU Haifang, PENG Juan, ZHAO Qiang, ZHANG Qiaoyu, HUANG Yaqin, ZHANG Zhiguo, SHEN Guanwang, QI Yuliang, WANG Quanxiu, SUN Yanfang. Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 26-34. doi: 10.7668/hbnxb.20195799.

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Figures/Tables 7

Tab.1 Primer sequences

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
hpt-F	ACACTACATGGCGTGATTTCAT
hpt-R	TCCACTATCGGCGAGTACTTCT
β-actin-F	TGCTATGTACGTCGCCATCCAG
β-actin-R	AATGAGTAACCACGCTCCGTCA
OsAAP8-F	CAACGTCTCCAGCGTGCCCTAC
OsAAP8-R	GGCTTCCCTTCAACCCTCC

Fig.1 Identification of OsAAP8 overexpressed transgenic positive plants A.Positive identification of OsAAP8 overexpressed plants:Marker.2 000 bp; B.OsAAP8 expression level detection. Different letters indicate significant difference(P<0.05).The same as Fig.2—6.

Fig.2 Detection and analysis of agronomic traits in OsAAP8 overexpressed transgenic plants A.Phenotypic observation of OsAAP8 overexpressed transgenic lines,the scale is 15 cm.

Fig.3 Detection and analysis of nutritional quality traits of OsAAP8 overexpressed transgenic rice

Fig.4 Detection and analysis of chalkiness traits in OsAAP8 overexpressed transgenic rice A.Statistical analysis of chalkiness traits;B.Observation of chalkiness under optical microscope(40×);C.Scanning electron microscopy observation of chalky areas;D.Scanning electron microscopy observation of non chalky areas.

Fig.5 Detection and analysis of grain size in OsAAP8 overexpressed transgenic rice

Fig.6 Detection and analysis of cooking taste quality and processing quality of OsAAP8 overexpressed transgenic rice

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