Abstract:

To clarify the effect of short-day photoperiod induction on the metabolic material changes in adzuki bean leaves,the late maturing variety Jihong 16 was used,and 10 h light /14 h dark short-day photoperiod induction was set.The group(10h14d)of short-day photoperiod induction and control group(CK)were studied using ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The results demonstrated that a total of 128 significantly differential metabolites were obtained by quantitative testing(103 substances were up-regulated and 25 substances were down-regulated),including 11 metabolites,such as flavonoids,amino acids and derivatives,organic acids,phenolic acids etc,the number(proportion)were 49(38.28%),26(20.31%),12(9.38%),10(7.81%),all above 10%.KEGG enrichment analysis found that 128 metabolic substances were enriched in 49 metabolic pathways.Moreover,significant differential top 20 metabolic pathways were mainly enriched in glucosinolate biosynthesis,aminoacyl-tRNA biosynthesis,2-oxocarboxylic acid metabolism,isoflavonoid biosynthesis,cyanoamino acid metabolism,biosynthesis of amino acids,flavonoid biosynthesis etc.Among the top 10 metabolic pathways with a proportion greater than 10%,the enriched metabolites were mainly acids and ketones.Moreover,most of these metabolites were upregulated.In summary,ketone and acid-related metabolites are considered to be main metabolites in response to short-day photoperiod induction.This provides a theoretical basis for optimizing the planting structure and improving the yield and quality of adzuki bean.

Key words: Adzuki bean, Short-day photoperiod induction, Metabolome, Metabolic pathway, Differential metabolites

CLC Number: 

• S521.01