Genome-wide Identification and Stress Expression Analysis of IQM Gene Family in Maize

doi:10.7668/hbnxb.20195645

Abstract

Abstract:

IQM(IQ-motif containing protein),a plant-specific calmodulin-binding protein,plays crucial roles in plant growth,development,and responses to various stresses.In order to study the characteristics and potential functions of the maize IQM gene family,bioinformatics approaches were used to identify IQM genes in the maize whole genome,and protein properties,phylogenetic relationship,gene structure,chromosome location,gene replication,cis-acting element,tissue-specific expression and expression patterns under various stresses were investigated.A total of 11 ZmIQMs genes were identified in the whole genome of maize,named ZmIQM1 to ZmIQM11 based on their chromosomal locations.ZmIQMs genes could be classified into three subfamilies,with genes within different subfamilies exhibiting similar structures.Segment duplication was found to play a major role in the amplification and evolution of the ZmIQMs gene family.Cis-acting element analysis showed that the promoter region of ZmIQMs gene contained multiple hormone and stress response elements.The expression pattern of ZmIQMs genes was investigated,and it was found that ZmIQMs genes had different expression patterns in different tissues,and the expression levels of several ZmIQMs genes were changed under different abiotic and biotic stresses.qRT-PCR results showed that under drought stress,the expression of ZmIQM3,ZmIQM4 and ZmIQM10 was up-regulated, and ZmIQM3,ZmIQM4,ZmIQM5,ZmIQM10 and ZmIQM11 responded to Cochliobolus heterostrophus infection.The results showed that ZmIQMs genes played an important role in stress response.

Key words: Maize, IQM, Gene identification, Gene expression pattern

CLC Number:

S513.03

WANG Ziming, SI Jihao, ZHANG Wenzhi, MENG Cheng, WANG Zhe. Genome-wide Identification and Stress Expression Analysis of IQM Gene Family in Maize[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(4): 1-9. doi: 10.7668/hbnxb.20195645.

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Figures/Tables 11

References 31

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基因 Gene	上游引物(5'-3') Forward primer (5'-3')	下游引物(5'-3') Reverse primer (5'-3')
ZmIQM3	CAGAGAGGCGTATTCCAGCA	TGAGGAGGTTCTCTGCACTAGG
ZmIQM4	ACGGCAGGGACTTGCATCA	TACACGAGCCGCCCTTCCT
ZmIQM5	CATCATTAGCGAGGGAAAGGT	CAGTGAATCTCCCAGCAGCTA
ZmIQM10	AAGATGACGATGATGGCCAG	GTAGTGCAGGCTGGGCGA
ZmIQM11	GCCCGACGGAGGAGAACTT	TGCCCCAGAACTCCTCGTC
ZmEF-1α	TGGGCCTACTGGTCTTACTACTGA	ACATACCCACGCTTCAGATCCT

基因 Gene	上游引物(5'-3') Forward primer (5'-3')	下游引物(5'-3') Reverse primer (5'-3')
ZmIQM3	CAGAGAGGCGTATTCCAGCA	TGAGGAGGTTCTCTGCACTAGG
ZmIQM4	ACGGCAGGGACTTGCATCA	TACACGAGCCGCCCTTCCT
ZmIQM5	CATCATTAGCGAGGGAAAGGT	CAGTGAATCTCCCAGCAGCTA
ZmIQM10	AAGATGACGATGATGGCCAG	GTAGTGCAGGCTGGGCGA
ZmIQM11	GCCCGACGGAGGAGAACTT	TGCCCCAGAACTCCTCGTC
ZmEF-1α	TGGGCCTACTGGTCTTACTACTGA	ACATACCCACGCTTCAGATCCT

基因名称 Gene name	基因ID Gene ID	基因位置 Genome location	开放阅读框长度/bp Open reading frame length	氨基酸数/aa Amino acids	分子质量 /ku Molecular weight	等电点 Isoelectric point	染色体 Chromo- some	亚细胞定位 Subcellular localization
ZmIQM1	Zm00001d027815	136597264-136600860	1 929	642	71.583 58	8.94	1	细胞核
ZmIQM2	Zm00001d032981	223073135-223075832	1 971	656	73.426 83	8.39	1	细胞核
ZmIQM3	Zm00001d004916	79837610-79839037	1 104	367	41.307 62	8.89	2	细胞核
ZmIQM4	Zm00001d044253	192080883-192084967	1 689	562	62.140 52	9.50	3	叶绿体
ZmIQM5	Zm00001d052525	173537172-173539940	1 407	468	52.064 74	8.58	4	细胞核
ZmIQM6	Zm00001d035377	14329827-14333778	1 311	436	49.035 75	9.16	6	叶绿体
ZmIQM7	Zm00001d037759	23680168-23690640	1 587	528	58.222 00	9.36	6	细胞核
ZmIQM8	Zm00001d022245	154193631-154195926	1 986	661	73.130 90	7.54	7	细胞核
ZmIQM9	Zm00001d009755	123978579-123979350	1 341	446	49.004 68	9.59	8	细胞核
ZmIQM10	Zm00001d011228	84015105-84017555	1 653	550	60.230 04	9.43	8	叶绿体
ZmIQM11	Zm00001d047402	156642268-156645976	1 617	538	59.073 49	7.60	9	细胞核

基因名称 Gene name	基因ID Gene ID	基因位置 Genome location	开放阅读框长度/bp Open reading frame length	氨基酸数/aa Amino acids	分子质量 /ku Molecular weight	等电点 Isoelectric point	染色体 Chromo- some	亚细胞定位 Subcellular localization
ZmIQM1	Zm00001d027815	136597264-136600860	1 929	642	71.583 58	8.94	1	细胞核
ZmIQM2	Zm00001d032981	223073135-223075832	1 971	656	73.426 83	8.39	1	细胞核
ZmIQM3	Zm00001d004916	79837610-79839037	1 104	367	41.307 62	8.89	2	细胞核
ZmIQM4	Zm00001d044253	192080883-192084967	1 689	562	62.140 52	9.50	3	叶绿体
ZmIQM5	Zm00001d052525	173537172-173539940	1 407	468	52.064 74	8.58	4	细胞核
ZmIQM6	Zm00001d035377	14329827-14333778	1 311	436	49.035 75	9.16	6	叶绿体
ZmIQM7	Zm00001d037759	23680168-23690640	1 587	528	58.222 00	9.36	6	细胞核
ZmIQM8	Zm00001d022245	154193631-154195926	1 986	661	73.130 90	7.54	7	细胞核
ZmIQM9	Zm00001d009755	123978579-123979350	1 341	446	49.004 68	9.59	8	细胞核
ZmIQM10	Zm00001d011228	84015105-84017555	1 653	550	60.230 04	9.43	8	叶绿体
ZmIQM11	Zm00001d047402	156642268-156645976	1 617	538	59.073 49	7.60	9	细胞核

同源基因对 Paralogous pairs	复制类型 Duplicate type	非同义替换率 Ka	同义替换率 Ks	非同义替换率/同义替换率 Ka/Ks	复制时间/Mya Duplication time
ZmIQM1-ZmIQM2	片段重复	0.283 2	1.172 5	0.241 6	26.517 9
ZmIQM3-ZmIQM5	片段重复	0.098 5	0.346 7	0.283 9	9.414 2
ZmIQM4-ZmIQM10	片段重复	0.107 2	0.207 2	0.517 6	32.840 1
ZmIQM8-ZmIQM11	片段重复	0.203 4	0.689 9	0.294 8	26.150 2

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