Effects of Exogenous Sucrose on Seed Germination and Seedling Development of Brassica napus L.

doi:10.7668/hbnxb.20195149

Abstract

Abstract:

Germination control is an important basis for promoting rapid emergence and uniform seedlings of Brassica napus.In order to explore the effect of exogenous sucrose on B.napus seed germination and seedling development,it selected the early-maturing rapeseed variety-Xiangyou 420 to conduct germination tests under different conditions and detected the expression changes of moisture,abscisic acid,endogenous sugars and key genes.The results showed that exogenous sucrose delayed the germination initiation process of Xiangyou 420 seeds,improved the uniformity of germination,promoted the cotyledons to turn green and formed true leaves after the cotyledons expanded,inhibited the excessive elongation of the main root and promoted the development of lateral roots.Exogenous sugar first inhibited and then promoted water absorption during the germination process of Xiangyou 420 seeds.It inhibited water absorption within three hours after sowing and released the inhibition of water absorption within six hours after sowing.The ABA content in seeds continued to decrease during the germination process,and exogenous sucrose promoted the decrease in ABA content within three hours after sowing.The total soluble sugar content decreased rapidly within 15 to 18 hours after sowing,and the reducing sugar content increased with the germination process in the early stages of germination.Exogenous sucrose had no significant effect on the changes in total soluble sugar content but inhibited the increase in reducing sugar content within nine hours after sowing and promoted the increase in reducing sugar content 15 hours after sowing.During the germination process,the expression of two sucrose phosphate synthase (BnaSPS) genes decreased significantly during the radicle germination stage and then increased after the cotyledons turned green.Exogenous sucrose inhibited the expression of the two BnaSPS genes before radicle germination, and significantly induced,the expression of BnaC09g37470D gene after the cotyledons turned green.In summary,it preliminarily reveals that exogenous sucrose regulates water absorption at the initial stage of imbibition through osmotic effects,and delays the initiation of B.napus seed germination by regulating endogenous sugar utilization in seeds during the germination stage and sugar conversion in the early stages of seedling development through sugar metabolism,promotes the seed germination process after imbibition,improves the uniformity of seed germination,and promotes the development of seedlings.

Key words: Brassica napus L., Seed germination, Seedling development, Exogenous sucrose, Gene expression

FENG Tao, CHEN Qinqin, YANG Jia, TAN Hui, YIN Mingzhi, HU Yan. Effects of Exogenous Sucrose on Seed Germination and Seedling Development of Brassica napus L.[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 110-116. doi: 10.7668/hbnxb.20195149.

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References 20

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引物名称 Primer	序列(5'-3') Sequence (5'-3')	目标基因 Gene
BnaActin_F	AACCACCTACAACTCCATAA	BnaActin(BnaA04g04550D)
BnaActin_R	CCTCCAATCCAGACAGAATA	BnaActin(BnaA04g04550D)
BnaSPS_C9_F	CGACTGACTACTGCTACAC	BnaC09g37470D
BnaSPS_C9_R	CACGAACACCACCATCTT	BnaC09g37470D
BnaSPS_A10_F	TCAACTGACTACTGCTACAC	BnaA10g15120D
BnaSPS_A10_R	CACGAACACCACCATCTT	BnaA10g15120D

引物名称 Primer	序列(5'-3') Sequence (5'-3')	目标基因 Gene
BnaActin_F	AACCACCTACAACTCCATAA	BnaActin(BnaA04g04550D)
BnaActin_R	CCTCCAATCCAGACAGAATA	BnaActin(BnaA04g04550D)
BnaSPS_C9_F	CGACTGACTACTGCTACAC	BnaC09g37470D
BnaSPS_C9_R	CACGAACACCACCATCTT	BnaC09g37470D
BnaSPS_A10_F	TCAACTGACTACTGCTACAC	BnaA10g15120D
BnaSPS_A10_R	CACGAACACCACCATCTT	BnaA10g15120D

处理 Test	萌发率/% Germination rate	起始露白时间/h Seed initial germination time	全部露白时间/h Total seed germination time
T0	100	15.20±0.25b	25.20±0.35a
T1	100	18.30±0.21a	25.00±0.18a
T2	100	15.25±0.35b	26.30±0.51a
T3	100	16.20±0.22b	25.10±0.34a

处理 Test	萌发率/% Germination rate	起始露白时间/h Seed initial germination time	全部露白时间/h Total seed germination time
T0	100	15.20±0.25b	25.20±0.35a
T1	100	18.30±0.21a	25.00±0.18a
T2	100	15.25±0.35b	26.30±0.51a
T3	100	16.20±0.22b	25.10±0.34a

基因ID Gene ID	染色体定位 Gene location	CDS长度/bp Length of CDS	分型 Type
BnaC09g37470D	C09	2 898	A
BnaA10g15120D	A10	2 898	A
BnaA02g04800D	A02	2 916	A
BnaA03g03230D	A03	3 117	A
BnaC03g04660D	C03	2 886	A
BnaC07g29460D	C07	2 901	A
BnaA06g27590D	A06	2 880	A
BnaA10g03060D	A10	3 192	B
BnaC05g02930D	C05	3 195	B
BnaC02g29300D	C02	1 419	C
BnaA02g23460D	A02	3 141	C

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