Abstract: To explore to study the effect of exogenous calcium on the tolerance of Leymus chinensis, the seeds which collected from Horinger County, Hohhot City, Inner Mongolia were used as experimental materials, and the method of aseptic culture was used to study the effect of CaCl₂ on the growth and physiological characteristics of Leymus chinensis under four abiotic stresses of salt, alkali, osmotic and cold stress. The results showed that the growth of Leymus chinensis seedlings was significantly inhibited under 150 mmol/L NaCl stress. After adding 1 mmol/L CaCl₂ to the culture medium, the germination rate of seeds was significantly increased, and the root length, leaf length and fresh weight of seedlings were also increased. Under pH 8.5 or 300 mmol/L Mannitol stress, the growth of Leymus chinensis seedlings was both significantly inhibited. The effects of alkali stress and osmotic stress on the growth of Leymus chinensis seedlings could not be effectively alleviated by adding 1-20 mmol/L CaCl₂ to the culture medium respectively. Under 4℃ stress, the growth of Leymus chinensis seedlings was significantly inhibited. After adding 20 mmol/L CaCl₂ to the culture medium, the leaf length and fresh weight of seedlings increased significantly, the content of malondialdehyde(MDA) in leaves decreased, the activity of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) and glutathione reductase(GR) increased, and the content of reduced ascorbic acid(AsA) increased significantly. In conclusion, the addition of exogenous CaCl₂ could improve the salt and cold resistance of Leymus chinensis seedlings, but the ability to resist alkali and osmotic stress has not been improved.

Key words: Leymus chinensis, Calcium, Abiotic stress, Growth characteristics, Physiological characteristics

CLC Number: 

• Q945.78