Tissue Expression Analysis of C1QA, C1QB and C1QC Genes in Yak of Three Ages

doi:10.7668/hbnxb.20191788

Abstract

Abstract: Complement C1q(complement 1q)protein consists of three polypeptide chains,A,B and C,C1q plays an important role in oxidative stress,cell apoptosis,and maintaining the stability of the internal environment of the organism.To explore the expression patterns in various tissues at different ages,and will lay the foundation for the further understanding of the functions of C1q proteins of yak.Total RNA were extracted from different tissues,including heart,spleen,lung,brain and cerebella.The mRNA expression levels of C1QA, C1QB,C1QC genes were determined by Quantitative Real-time PCR(RT-qPCR).SPSS 22.0 software was used to analyze the significant difference between different tissues.RT-qPCR results indicated that C1QA, C1QB and C1QC genes were expressed in all tissues.The expression trend of different age groups was basically the same.The expression levels in lung of C1QA,C1QB and C1QC genes were significantly higher than spleen,heart,brain and cerebella(P<0.05);in spleen was significantly higher than heart,brain and cerebella(P<0.05);in heart was significantly higher than brain and cerebella(P<0.05),and there was no significant difference in the expression level between brain and cerebella(P >0.05).The results also showed that the expression levels of C1QA, C1QB and C1QC genes were the highest in 1.5-year-old yak lungs,which were significantly higher than those in spleen,heart,brain and cerebella(P<0.01).With the growth of the yak, C1QA gene expression level in most tissues was decreased significantly.In stark contrast,we found that gene expression level of C1QC was increased significantly in all tissues.While,the mRNA expression of C1QB gene was dynamic,with the level dramaticlly increasing and then significantly decreased with aging.In a word,in the high-altitude adaptationregulation of yak, C1QA,C1QB genes maybe involved through the down-regulation of tissue expression level,and C1QC maybe through the up-regulation.

Key words: Yak, C1q, Tissue expression, Temporal expression, High-altitude adaptation

CLC Number:

Q78
S823

ZHONG Mei, WANG Jikun, CHAI Zhixin, WU Zhijuan, ZHONG Jincheng. Tissue Expression Analysis of C1QA, C1QB and C1QC Genes in Yak of Three Ages[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 385-391. doi: 10.7668/hbnxb.20191788.

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