Identification and Salinity Stress-responsive Analysis of PP2C Genes in Peanut

doi:10.7668/hbnxb.2018.03.012

Abstract

Abstract: In order to elucidate the role of protein phosphatase 2C genes (PP2C) in peanut,RNA sequencing (RNA-Seq) was conducted with leaves of a salinity tolerant peanut mutant (S2) and its control (S4) before and after salinity treatment as materials in this experiment. 79 PP2C genes with complete open reading frame were screened to distribute on 10 chromosomes of A genome,the gene number of each chromosome was significantly different with the variation range of 1 to 15. According to the clustering result of PP2C genes from Arabidopsis,these 79 PP2C genes from peanut were divided into 12 subgroups and 15 subkinds. To analyze the response to salinity stress of these 79 PP2C genes,digital gene expression profiles were constructed with S2 and S4 before and after salinity treatment. The results showed that 35 PP2C genes belonging to 12 subgroups and 14 subclasses were responsive to salinity stress including all the members belonging to b subclass of A subgroup,b subclass of G subgroup,I subgroup and L subgroup;while all the members belonging to a subclass of G subgroup were insensitive to salinity stress. Some important stress-responsive elements were found in promoters of the most of these 35 PP2C genes,such as heat shock element (HSE),MYB binding site involved in drought inducibility (MBS),stress responsive element TC-rich repeats and antioxidant-response element (ARE),etc. The results could provide basis for functional research and the application of PP2C genes in peanut.

Key words: Peanut, PP2C gene, Salinity stress, cis-regulatory element

CLC Number:

Q785

CHEN Guanxu, QIN Guilong, LI Enguang, ZHAO Chunmei, QIAO Lixian, WANG Jingshan, SUI Jiongming. Identification and Salinity Stress-responsive Analysis of PP2C Genes in Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 71-77. doi: 10.7668/hbnxb.2018.03.012.

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