Abstract: To reveal the function of rice polypeptide release factor eRF1 in protein synthesis,the full length cDNA and promoter (2 120 bp) of rice eRF1 gene,named OseRF1-3,was isolated by PCR.Sequence analysis indicated that the OseRF1-3 contained a 1 308 bp CDS sequence that encoded 436 amino acids with three conserved N,M and C domains.qRT-PCR results indicated that the transcript of OseRF1-3 was detected in all of the tissues examined,but the highest level of expression was detected in spikes.The genome DNA from leaves of rice was used as the template, the 2 120 bp promoter sequence of the initiation codon upstream of OseRF1-3 gene were cloned using the PCR method. The promoter of OseRF1-3 gene was fused with GUS reporter gene and transferred into Oryza sativa L.callus by Agrobacterium-mediated method.Histochemical staining of different organs of the transgenic plants showed that histochemical GUS expression could be found in all of the tissues examined,and a low GUS reporter gene expression in root and higher expression in flowers and rice husk.The results would provide the basis for the understanding of the OseRF1-3 gene function.The results of GUS gene expression driven by the promoter of OseRF1-3 gene were in good agreement with the results from qRT-PCR analysis. So the results will provide the basis for the further explore of the OseRF1-3 gene function.

Key words: Rice, Polypeptide release factor, Promoter, Expression pattern, GUS

CLC Number: 

• S511.03

• Q78